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| Molecular Epidemiology of Pathogen Streptococcus agalactiae from Tilapia in Southern China |
| WANG Bei,LI Yuan,CHEN He,LI Gui-huan,LU Yi-shan,TANG Ju-feng,CAI Jia,WU Zao-he,JIAN Ji-chang |
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Abstract Identification was carried out in 128 strains of Streptococcus isolated from tilapia in southern China (Guangdong ,Guangxi and Hainan provinces ) from 2010 to 2011 by Bio‐MerieuxVITEK and 16S rRNA sequence analysis and 7 housekeeping genes were seguenced in 104 strains of S .agalactiae by Mul‐tilocus Sequence Typing (MLST ) technique to evaluate the genetic diversity of S . agalactiae .Results were analyzed and compared with sequence information in PubMLST databases to determine a strain allelic profile and assigned a sequence type (ST ) .It was found that total 104 strains were within 3 clone se‐quences :ST‐7 and 2 types of unidentified NST‐1 and NST‐2 ,NST‐1 having the maximal proportion of 88 .5% (92/104) .It was revealed that there were multiple clonal sequences of S .agalactiae isolate from tilapia in southern China with high pathogenetic and prevalent .It is suggested that S . agalactiae isolate from tilapia be intense surveillance efforts .The findings indicate that MLST technique is an ideal tool to reveal genetic evolution profile of S .agalactiae isolated from tilapia in southern China .
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Received: 25 December 2014
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