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| Screening and Antagonistic Conditions of Antagonistic Bacteria Against Vibrio parahaemolyticus |
| YANG Sheng-yuan,WEI Jin |
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Abstract The strain JNT02 ,which showed significantly inhibitory effects on the growth of Vibrio parah‐aemolyticus with (20 .87 ± 0 .83) mm diameter of antibiotic circle fermented broth for antimicrobial activity assay ,was screened from 42 bacterial strains isolated from vegetable garden soils ,garlic plants ,soybean plants ,vine peanut plants ,freshwater lake silts ,cactus plants ,celery plants ,bacterial soybean lobster sauce and Japanese natto especially producing antibiotic substances against Escherichia coli .The strain JNT02 was classified into Bacillus subtilis base on 16S rDNA sequence and phylogenic analysis .In the im‐proved Landy media ,B .subtilis JNT02 completely killed the co‐cultured V .parahaemolyticus in 24 h , and the enhancement of antagonistic effects of the antimicrobial substances brought from the B .subtilis JNT02 seed broth were also demonstrated .However ,B .subtilis JNT02 showed weak inhibitory effects a‐gainst the growth of V . parahaemolyticus in alkaline‐peptone‐water media and no antagonistic effects in artificial seawater .The improved Landy media were propitious to the antimicrobial substance production of B .subtilis JNT02 .The findings indicated that the antagonistic action of B .subtilis JNT02 against V . parahaemolyticus was via the antimicrobial substance producing ,and depended on the conditions of the an‐timicrobial substance production ,in stead of the growth of B .subtilis JNT02 .
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Received: 25 December 2014
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