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| cDNA Sequence and Expression Analysis of IgL3 Gene in Yellow Catfish, Pelteobagrus f ulvidraco |
| LI Chun-tao,JIANG Zi-Li,WEI Fu-lun,YANG Xiu-rong |
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Abstract The technique of homologous cloning and RACE was used to amplify full length cDNA of IgL 3 gene from yellow catfish (Pelteobagrus f ulvidraco) .IgL3 in yellow catfish has 965 nucleotides ,including 5′‐UTR of 53 nucleotides ,3′‐UTR of 189 nucleotides and an open reading frame with 723 nucleotides en‐coding a peptide of 241 amino acids .The IgL3 comparison in seven teleost species showed that IgL 3 in yellow catfish shared the maximal identity (72 .8% ) with that in Mystus macropterus ,and the minimal i‐dentity(42 .2% ) with that in Atlantic salmon Salmo salar .Phylogenetic tree based on some teleost IgL a‐mino acids showed that IgL3 in yellow catfish was clustered closely with that of M .macropterus and IgL3 was far away from L1 and L2 of teleost .Real‐time PCR revealed that IgL3 mRNA expression of yellow catfish was mainly detected in head kidney ,spleen ,blood cells ,gill and intestine and increased significant‐ly in these tissues after injection of Aeromonas hydrophila .The results indicated that these tissues were main responding organs for IgL3 expression after stimulation ,and played a critical role in immunity inter‐action in yellow catfish .
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Received: 25 December 2014
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