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| Isolation and Identification of Pathogen Proteus mirabilis from Diseased Chinese Soft-shelled Turtle Pelodiscus sinensis |
| LIN Ya-ge,YE Jian,SHI Ting-ting,SONG Yang,WANG Yu-feng,ZHANG Xiao-dong |
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Abstract One bacterium was isolated from liver and ascites of diseased Chinese soft‐shelled turtle (Pelo‐discus sinensis ) ,outbreaked in a large‐scale breeding farm in Shangyu ,and identified as Proteus mirabilis , named SX37 through API ID32E by 16S rRNA sequencing , and biochemical reactions . Healthy soft‐shelled turtle was experimentally infected with the bacterial suspension ,and then the same bacterium was recovered with different clinic sydromes .Isolate SX43 demonstrated virulence potential in mouse models , with the median lethal dose (LD50 ) of 1 × 107 .83 cfu/mL in mice .
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Received: 25 December 2014
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