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| Single and Joint Acute Toxicity of Cd2+ and Hg2+ to Chinese Mitten Handed Crab Eriocheir sinensis |
| PENG Xue,HUANG Xiaoliang,CHEN Sihan,CHEN Shangjing,YING Xueping |
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Abstract Chinese mitten handed crab Eriocheir sinensis with body weight of 48 .58—87 .09 g was exposed to Cd2+ concentrations of 25 .00 ,52 .23 ,109 .13 ,228 .00 ,476 .37 and 993 .27 mg/L and Hg2+ concentra‐tions of 3 .00 ,7 .26 ,17 .58 ,42 .57 ,103 .06 and 249 .53 mg/L ,respectively ,and jointly by mixing equitoxic ratio of 96 h‐median lethal concentrations (LC50 ) of Cd2+and Hg2+ for 4 days to understand the acute tox‐icity effect of Cd2+ and Hg2+ on survival of the crab .The results showed that the LC50 of Cd2+ were 654 .226 mg/L in 24 h ,192 .429 mg/L in 48 h ,70 .767 mg/L in 72 h ,and 40 .279 mg/L in 96 h ,and the LC50 of Hg2+ were 164 .157 mg/L in 24 h ,19 .214 mg/L in 48 h ,6 .738 mg/L in 72 h and 3 .641 mg/L in 96 h .The safety concentrations (SC) were shown to be 4 .994 mg/L for Cd2+and 0 .079 mg/L for Hg2+ , higher toxicity in Hg2+ than in Cd2+ .The joint toxicity test revealed that the additive index (AI) was 4 .291 in 24 h ,4 .587 in 48 h ,3 .367 in 72 h and 2 .003 in 96 h for equitoxic mixtures of Hg2+ and Cd2+ , showing the synergistic joint toxic effects of Cd2+ and Hg2+ .
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Received: 25 April 2015
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