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| Effects of Handling Stress out of Water on Serum Parameters in Tiger Puffer Taki f ugu rubripes |
| CHENG Minjie,HUANG Yadong,XU Wenmin,WAN Gangtao,XING Kezhi,CHEN Chengxun,WANG Qingkui,GUO Yongjun,SUN Xueliang |
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Abstract The serum indices were determined 0 ,4 ,8 ,12 ,18 ,24 ,36 ,48 ,72 ,96 ,120 ,and 168 h after handling out of water in tiger puffer Taki f ugu rubripes with body weight of (181 .88 ± 14 .95) g handled out of water (out of water for 3 min ,in water for 2 min ,and then out of water for 3 min ) at water temperature of (22 .0 ± 0 .3) ℃ .The results showed that activities of glutamate‐pyruvate transaminase (GPT) and glutamate‐oxaloacetate transaminase (GOT) were found to be increased after handling ,GPT and GOT being recovery to the control level 72 h and 8 h later ,respectively .The activities of superoxide dismutase (SOD) and catalase (CAT) were increased first and then decreased gradually ,being recovery to the control level 12 h later .The maximal CAT activity was observed 18 h after handling ,and returned to the control level after 48 h ,significantly higher than that in the control group from 8 h to 24 h later (P<0 .05) .There was higher content of malonaldehyde (MDA) in the fish in the treatment group than in the control group during the test .The levels of creatinine (Cr) and urea nitrogen (BUN) were found to be increased after handling ,and then returned to the control level . The activity of alkaline phosphatase (AKP) was found to be significantly lower than that in the control group after handling (P<0 .05) ,AKP being recovery to the control level .There was no significant difference in content of total protein (TP) between the handling group and the control group( P>0 .05) .The findings indicate that the handling out of water might damage liver and kidney due to unbalancing and disorder .The handled fish will returned to the normal level 72 h after handling .
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Received: 25 April 2015
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