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| Cloning,Characterization and Expression of Full-length cDNA of a Tyrosinase Gene from Japanese Scallop Patinopecten yessoensis |
| SHI Jingyun,BAO Xiangbo,ZHANG Zhenyu,GAO Xianggang,PU Hongshuang,HE Chongbo,KONG Fandong,LIU Weidong |
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Abstract In this study ,tyrosinase (MyTYR) gene was sequenced from Japanese scallop Patinopecten yessoensis by molecular cloning technique ,and MyTYR expression was studied in different tissues in the Japanese scallop challenged with bacterial pathogen (Vibrio anguillarum) at various periods of injection by quantitative real time PCR (qRT‐PCR) .The full‐length 1850 bp MyTYR mRNA contained a 37 bp 5’ untranslated region ,a 1470 open reading frame encoding 489 amino acids and a 343 bp 3’ UTR .The qRT‐PCR showed that MyTYR gene was expressed in the hepatopancreas ,mantle ,kidney ,adductor ,gill and hemocytes ,the maximum in the mantle .There was significantly lower quantity of TYR gene expression 6 hours after the bacterial pathogen injection than that in the control group .From twelve to thirty‐six hours after injection ,however ,the expression level of TYR gene was significantly higher than that in the control group ,indicating that the gene may play an important role in immune process .
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Received: 25 April 2015
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