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| Cloning and Phylogeny Analysis of GnRHs in Croaker Nibea Protonibea diacanthus |
| YANG Xiankuan,ZHAO Huihong,LIN Quanzhuo,CHEN Yibin,LIU Lijie,LIU Yan,LI Zhihu |
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Abstract Three genes of gonadotrophin hormone releasing hormone (GnRHs) including cGnRH (chicken GnRH) ,(salmon GnRH) and sbGnRH(sea bream GnRH) were cloned from croaker nibea Protonibea di‐acanthus by a homology cloning method .It was found that the ORF was comprised of 258 bp coding 85 a‐mino acids in cGnRH ,273 bp coding 90 amino acids in sGnRH and 261 bp coding 86 amino acids in sbGn‐RH .T he three GnRH genes had signal peptide of 23 amino acids ,and the highly conservative core de‐capeptide of QHWSHGWYPG in cGnRH ,QHWSYGWLPG in sGnRH and QHWSYGLSPG in sbGnRH by bioinformatics analysis .Amino acid sequences alignment analysis proved that the amino acid sequences of cGnRH ,sGnRH and sbGnRH had the most affinity between croaker nibea and red drum Sciaenops ocel‐latus ,with the homology of up to 100% in the amino acid between cGnRH and sGnRH ,and the homology of 92 .2% in sGnRH .Phylogenetic unrooted trees constructed by neighbour‐joining method and the molec‐ular phylogenetic tree of GnRH established by NJ method using software MEGA 4 .0 showed that cGnRH of croaker nibea tended to cluster with red drum in the same family firstly ,then with puffer Taki f ugu ni‐phobles ,zebrafish Danio rerio ,and rainbow trout Oncorhynchus mykiss ;while that sGnRH tends to clus‐ter with sGnRH ,cGnRH and sbGnRH of other fish species step by step .The sbGnRH of croaker nibea was found to cluster with sbGnRH first ,followed by cGnRH ,and sGnRH successively .
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Received: 25 April 2015
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