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| Synergistic Effects of Temperature and Salinity on Activities of Amylase and Na+ /K+-ATPase in Juvenile Scallop Chlamys nobilis (Reeve) |
| QIAN Jiahui,LI Zhimin,CHEN Shuhe |
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Abstract The synergistic effects of temperature (19—31 ℃) and salinity (22—38) on activities of amylase and Na+ /K+-ATPase were studied in juvenile scallop Chlamys nobilis with shell length of (7 .52 ± 0 .12) mm by two-factor central composite design (CCD) and response surface methodology (RSM ) .There were significant linear and quadratic effects of temperature on the activities of amylase and Na + /K+-A T Pase ( P<0 .05) .The significant linear effect of salinity on the activity of Na + /K+-ATPase was found (P<0 .05) ,without significant linear effect of salinity on the activity of amylase (P>0 .05) ,and the significant quadratic effects of salinity on the activities of amylase and Na + /K+-ATPase were observed (P<0 .05) . There were significant synergistic effects of both temperature and salinity on the activity of amylase (P<0 .05) ,and no significant synergistic effects of both temperature and salinity on the activity of Na + /K+-ATPase were observed . The activities of amylase and Na+ /K+-ATPase were inhibited under high temperature and salinity ,with gradual decline in activities of amylase and Na + /K+-ATPase .A model equation about the relationship between the activities of amylase and Na + /K+-ATPase and the two factors was established by response surface methodology ,with the r2 as high as 0 .9815 in temperature and 0 .9582 in salinity , indicating that the satisfactory fitting capability of the model was practicably applied for prediction .The optimization of the reliable model revealed that the maximum activities of amylase (0 .4809 U/mg) and Na+ /K+-ATPase (0 .0382 U/mg) ,with the desirability value of 0 .910 ,were observed at the 2-factor combination of water temperature of 25 .60 ℃ and a salinity of 30 .67 .
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Received: 25 May 2015
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