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| Effects of BTEXs on Expression of Glutathione Peroxidase Gene in Intestine and Respiratory Tree of Sea Cucumber Apostichopus j aponicus |
| DONG Ying,WANG Bai,CUI Cheng,LI Shuman,GAO Shibo,GAO Shan,GUAN Xiaoyan,JIANG Bei,XHOU Zunchun |
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Abstract The present study is aimed to evaluate the effects of six kinds of BTEXs (benzene ,toluene , ethyl benzene ,o-xylene ,m-xylene and p-xylene) on the relative expression levels of glutathione peroxidase (GPx) gene in the respiratory tree and intestine of sea cucumber Apostichopus japonicus ,which is an important benthic organism in the Yellow Sea and the Bohai Sea of China ,using quantitative real-time PCR method . The results showed that the relative expression levels of GPx gene in respiratory tree and intestine were remarkably changed in different BTEXs treatment groups , and showed dose-effect relationship .The relative expression levels of GPx gene in respiratory tree was down-regulated in the benzene ,o-xylene and p-xylene treatment groups ,from 2 .6-fold to 41 .7-fold ;and up-regulated in the toluene and m-xylene treatment groups ,from 2 .6-fold to 86 .4-fold .The relative expression levels of GPx gene in intestine was down-regulated in the o-xylene ,m-xylene and p-xylene treatment groups ,from 1 .2-fold to 38 .5-fold .This suggested that the BTEXs had oxidative stress on the respiratory tree and intestine of the sea cucumber ,and might have oxidative damage in the two tissues .
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Received: 25 May 2015
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