|
|
|
| Effects of Vitamin C on Gene Expression of Transferrin during Early Development of Crucian Carp Carassius auratus gibelio |
| XIONG Hualong,YAO Junjie,JIANG Zuoyu,AN Miao,ZHU Zhongsheng |
|
|
|
|
Abstract In order to discuss the gene expression characteristics of transferrin and effects of vitamin C so‐lution on the expression of transferrin genes during early development of crucian carp Carassius auratus gibelio .RT‐PCR and fluorescence quantitative PCR techniques were used to detect effect of vitamin C on the expression of transferrin genes and its mRNA expression levels during the early development of crucian carp .The results showed no expression of transferrin in mature eggs ,fertilized eggs and expanded blasto‐cyst stage ,while the expression of transferrin was determined in gastrula stage ,and the mRNA expression level of transferrin showed “up‐down‐up” trend ,and a peak in the heatbeating stage ,and the minimum in the prehatching stage .In the group of vitamin C (30 mg/L) ,the mRNA expression level of transferrin was lower than that in normal group during embryonic stage ,while higher than that in normal group during larval stage .The findings showed that the function of transferrin was independent from the binding iron function during embryonic development ,and appropriate vitamin C led to be decreased in the function of transferrin in immune during embryonic development ,and to be increased in the function of transport iron in yolk‐sac larval stage .
|
|
Received: 25 June 2015
|
|
|
|
|
|
|
