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| Isolation,Identification and Antibiotic Susceptibility Testing of Plesiomonas shigelloides from Goldfish |
| ZHANG Pei,ZHU Aihua,HU Xiucai,LAN Yun,LI Xue,SHEN Xiaojing,L Aijun |
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Abstract A bacterium was isolated from hepatopancreas in goldfish (Carassius auratus)and named ZP‐1 . The isolate was identified by bacterial morphology ,physical and chemical characteristics ,16S rDNA se‐quencing and phylogenetic analysis .The results showed that ZP‐1 strain is Gram‐negative short‐rod shaped bacillus ,which was positive for acid production from glucose and lactose ,reduction of nitrate ,and indole production .The 16S rDNA of ZP‐1 strain was amplified by PCR ,and subcloned into pMD18‐T vector , whose sequencing was 1464 bp in length (accession number :KM458852) .Phylogenetic tree showed that strain ZP‐1 had high homology of 99 .80% with fish pathogenic Shiga Plesiomonas (KC825322) indicating that the isolate ZP‐1 was identified as P .shigelloides .The susceptibility testing was performed using 30 kinds of antibiotics ,and results showed that strain ZP‐1 was sensitive to cefotaxime ,cefixime ,cefaman‐dole ,gentamicin ,amikacin ,nalidixic acid ,chloramphenicol and ofloxacin .
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Received: 25 June 2015
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