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| Optimization of Ultrasound-assisted Autolysis and Exogenous Hydrolysis in Jinjiang Oyster(Crassostrea rivularis) |
| TIAN Yaqing,ZHENG Hua,LIN Jie,CHEN Zhikai,LI Shuying,WANG Yanfang |
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Abstract Ultrasound and protease can improve yields of short peptide by destroying the structure of protein .The effect of ultrasound power and time on autolysis of Jinjiang oyster (Crassostrea rivularis) was invesigated firstly to obtain high short peptide content considering hydrolysis degree and total volatile basic nitrogen .Then single‐factor experiment was conducted to evaluate effect of alkaline hydrolysis on the ultrasound‐assisted autolysis hydrolysate .The optimum ultrasound pretreated conditions were ascertained as 400 W ,and 10 min ,with short peptide yield of up to (88 .75 ± 1 .16) mg/mL ,2 .12 times as the control group .The total volatile basic nitrogen level was reduced to (79 ± 2) μg/g with hydrolysis degree of (30.38 ± 0 .05 )% . After ultrasound‐assisted autolysis , the hydrolysis conditions with alkaline were optimized ,and the results showed that enzyme concentration of 5 kU/g ,temperature 55 ℃ ,and for 4 h led to short peptide content of above 100 mg/mL ,with hydrolysis degree of (35 .95 ± 0 .05)% and total volatile basic nitrogen of (178 ± 6) μg/g ,which was in an acceptable range .The findings indicate that using ultrasound‐assisted autolysis with exogenous hydrolysis can significantly improve efficiency of enzymolysis .
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Received: 25 July 2015
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