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| Changes in Activity and Distribution of Cathepsin L-Like Proteases in Autolytic Octopus (Octopus ocellatus) |
| LIANG Xiaowang,ZONG Yuan,LIN Zhuyi,LI Dongmei,ZHOU Dayong |
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Abstract The contents of water‐soluble total proteins and collagen proteins ,as well as the activities of endogenous total proteases and cathepsin L‐like proteases were determined in arm muscle of the autolytic octopus induced by ultraviolet (UV ) . Results showed that the octopus became insensitive after UV irradiation .The body of octopus became softened ,and mucoid degeneration took place in epidermis and subcutaneous tissue .The changes in external appearance indicated that autolysis took place .Along with autolysis ,the contents of water‐soluble total proteins and collagen proteins were found to be increased , indicating degradation of structural proteins .At the same time ,the activities of endogenous total proteases and cathepsin L‐like proteases tissue were increased in tissues in wnit mass .The distribution of cathepsin L‐like proteases in arm muscle of the autolytic octopus was in homogeneous . The stained spots of cathepsin L‐like proteases were dense in myocardial trabeculation ,but were relatively sparse in muscle fibers .Along with the progress of autolysis ,the stained spots of cathepsin L‐like proteases were scattered and eventually disappeared ,indicating the dispersion of cathepsin L‐like proteases in tissue .The findings indicated that the endogenous proteases , cathepsin L‐like proteases as representive , took part in the autolysis of octopus .
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Received: 25 July 2015
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