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| Effects of Partial Replacement of Fish Oil by Soybean Oil on Serum Lipid Metabolism and Related Enzyme Activities in Chinese Striped-necked Turtle Mauremys sinensis |
| QIU Zhengyuan,SHENG Cheng,HONG Meiling,SHI Haitao |
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Abstract In order to study the effects of partial replacement of fish oil by soybean oil on serum lipid metabolism and the activity of correlated lipases . Four‐year old female Chinese striped‐necked turtle Mauremys sinensis [body weight(1557 ± 307) g] were randomly divided into four groups according to and fed the diets containing various proportions of fish oil (F) and soybean oil (S) including 0S∶3F (S∶F , 0∶3) ,1S∶2F (S∶F ,1∶2) ,2S∶1F(S∶ F ,2∶1) and 3S∶0F (S∶ F ,3∶0) for eight months .Six turtles were randomly selected from each group and determined for the content of triglycerides (TG) ,total cholesterol (TC) ,high density lipoprotein cholesterol (HDL‐C) and low density lipoprotein cholesterol (LDL‐C) in blood collected from the heart and the activity of lipoprotein lipase (LPL ) ,hepatic lipase (HL) and hormone sensitive lipase (HSL) in liver ,and pancreatic lipase (PL) in intestine .The results showed that the content of TC was significantly higher in 1S∶2F and 3S∶0F than that in 0S∶3F (P<0.05);the content of LDL‐C was significantly higher in 3S ∶ 0F than in 0S ∶ 3F ( P < 0 .05 ) , no significant differences in the other groups . The maximal activity of the total lipase (LPL + HL ) was observed in 2S∶1F ,significantly higher than that in the 0S∶3F group(P < 0 .05) and almost the same in HSL in 2S ∶ 1F to 0S ∶ 3F compared to the rest groups of HSL . There was no was not significant differentce in PL in the group of 2S∶1F from that in 0S∶3F ,but both being lower than that in the other groups .In totally of enzyme activity ,the group of 2S∶1F was also the suitable group .Above all ,the substitution of fish oil by soybean oil in the turtle could be worked ,with the suitable ratio of 2S∶1F .
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Received: 25 July 2015
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