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| Effects of Glycerol on Growth and Polysaccharides Accumulation of Alga Isochrysis zhanj iangensis |
| YANG Haibo,WANG Qian,CAO Xupeng,XUE Song |
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Abstract Effects of glycerol concentration (41 mmol/L ,110 mmol/L and 206 mmol/L ) on grow th and polysaccharides accumulation of alga Isochrysis zhanjiangensis were investigated in rich - nitrogen and limited-nitrogen culture systems by means of single factor experiments .The results showed that the cell density ,content and yield of polysaccharides were higher in 41mmol/L glycerol than those in the other glycerol concentrations . T he cell density , content and yield of polysaccharides in rich‐nitrogen culture system were 7 .73 × 107 cells/mL ,16 .21% and 0 .32 g/L ,respectively ,while those in limited‐nitrogen culture system were 5 .29 × 107 cells/mL ,54 .43% and 1 .02 g/L ,respectively .These data explained that lower glycerol concentration and higher nitrogen concentration were advantageous to the growth of I . zhanjiangensis (the biomass in the former was 1 .46 times as that in the later) ,however ,when glycerol concentration and nitrogen concentration were lower at the same time , the cell accumulated more polysaccharides (the content and the yield of polysaccharides of the later were 3 .36 times and 3 .19 times as the former ,respectively ) .No matter the difference in nitrogen supply ,with the increasing concentration of glycerol ,the cell density and polysaccharides content of I .zhanjiang‐ensis showed a decreasing trend . It indicated that high concentration of glycerol inhibited the growth of I .zhangjiangensis and the accumulation of polysaccharides .So ,I .zhangjiangensis should be cultured in the limited‐nitrogen culture system with low concentration of glycerol at inoculation to promote the accumulation of polysaccharides . The findings will provide reference with producing natural products of high value .
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Received: 25 August 2015
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