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| Effects of Photoperiods on Biomass,Astaxanthin Accumulation and Antioxidative Capacity in a Green Alga Haematococcus pluvialis |
| JIANG Hongxia,LIN Xiongping,LEI Mengyun,KONG Xianghui |
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Abstract The effects of photoperiods (0L∶24D ,4L∶20D ,8L∶16D ,12L∶12D ,16L∶8D ,20L∶4D and 24L∶0D) on biomass(cell density and dry weight) ,astaxanthin content(astaxanthin content perunit dry weight and astaxanthin content per cell) ,malondialdehyde content and total antioxidant capacity were investigated in green alga Haematococcus pluvialis . The biomass was determined by microscopic cells counting method and direct weighing method , and the astaxanthin content , malondialdehyde content and total antioxidant capacity were measured by a spectrophotometric method .The results showed that the biomass and malondialdehyde content were first increased and then decreased ,whereas astaxanthin content and total antioxidant capacity increased with the extension of daily illumination time .The biomass and malondialdehyde content reached the maximum values under photoperiod of 16L ∶ 8D ,and were significantly higher than those under continuous darkness and continuous illumination (P< 0.05) .The maximal astaxanthin content and total antioxidant capacity were obtained under continuous illumination ,and were significantly higher than those under illuminations of less than 20 hours per day (P<0.05) .There was no significant difference in biomass ,astaxanthin content per cell and malondialdehyde content between the two groups of algae under photoperiod of 20L∶4D and continuous illumination(P>0 .05) , whereas there was significant difference in astaxanthin content per dry weight and total antioxidant capacity between these two groups of algae (P<0 .05) .The biomass ,astaxanthin content ,malondialdehyde content and total antioxidant capacity were the minimum under continuous darkness .The findings provide the theoretical basis with the mass culture of H .pluvialis and the large‐scale production and application of astaxanthin .
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Received: 25 September 2015
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