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| Isolation,Identification and Effect of a Bacillus Strain on Immunity in Sea Cucumber Apostichopus j aponicus |
| GUAN Xiaoyan,CHEN Zhong,ZHAO Chunxia,GAO Shan,JIANG Jingwei,WANG Bai,JIANG Bing,LI Lili,GU Ying,DONG Ying |
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Abstract A dominant strain B4 isolated from the sediment of a healthy sea cucumber A postichopus j aponicus farm in Dalian was identified as Bacillus by morphological and physiological characteristics and 16S rDNA sequence analysis .The optimal growth of strain B4 were observed at temperature of 20—35 ℃ , pH of 6—9 and a salinity of 3—7 .5 .Then the activities of acid phosphatase (ACP) ,alkaline phosphatase (AKP) ,superoxide dismutase (SOD) ,lysozyme (LSZ) and phenoloxidase (PO) were detected in the coelomic fluids of the sea cucumber exposed to the strain B4 at concentration of 108 cfu/mL on 0 ,7 ,14 ,21 and 28 day after administration .The results showed that there were significantly higher AKP ,SOD and PO activities in the treatment groups than those in the control group 7 day after administration (P<0 .05) , while the coelomic fluid LSZ activity was higher at the 21th day (P<0 .05) .There was very significant difference in ACP activity on the 28th day between the control group and the test groups (P<0 .01) .The findings indicated that strain B4 led to improve the activities of AKP ,ACP ,PO ,SOD and LSZ in coelomic fluid of sea cucumber ,thus to have certain effect on immunity system .
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Received: 25 September 2015
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