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| Culturable Bacterial Diversity in Intestine of Farmed Juvenile Puffer Fish Taki f ugu rubripes |
| LI Yanyu,REN Meng,ZHANG Congyao,WANG Lianshun,ZHU Ying,MA Yuexin |
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Abstract It w as aimed to examine the bacterial community associated to intestinal mucus of farmed juvenile puffer fish Takifugu rubripes .Polymerase chain reaction and sequencing of partial 16S rDNA were performed on DNA from bacteria cultivated on Zobell 2216E .All the isolates were classified into two phyla , Proteobacteria and Firmicutes . Gamma‐Proteobacterium comprised the dominant culturable intestinal microbiota (83 .3% ) ,and 8 genera of bacteria were isolated from the intestine ,including V ibrio , Shewanella,Pseudoalteromonas,Sulfitobacter,Bacillus,Aliivibrio,Photobacterium,and Colwelliain which Vibrio , Shewanella ,and Pseudoalteromonas accounted for 70% of the total . Sulf itobacter and Colwellia were the first reported as the intestinal bacterial microflora in puffer fish T .rubripes .
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Received: 25 October 2015
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