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| Antioxidant Enzyme System in Gills of Shrimp Exopalaemon carinicauda Challenged with Vibrio anguillarum |
| DUAN Yafei,LIANG Junping,LI Jitao,WANG Yun,ZHANG Zhe,LI Jian,LIU Ping |
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Abstract The total antioxidant capacity and the mRNA expression levels of glutathione peroxide reduc‐tase ,catalase ,superoxide dismutase ,peroxiredoxin and glutathione S‐transferase genes w ere investigated in gills of healthy shrimp Exopalaemon carinicauda with body weight of (1 .18 ± 0 .35) g injected with sterile 0.86% saline solution and bacterium V ibrio anguillarum 0 ,3 ,6 ,12 ,24 ,48 ,and 72 h after injec‐tion to study the effect of V . anguillarum challenge on the antioxidant enzyme defense system of the shrimp .The results showed that the total antioxidant capacity in gills were found to be increased signifi‐cantly in the shrimp challenged with V .anguillarum ,to the peak value 6 h after administration ,and then decreased gradually to a lower level than in the shrimp in the control in 48 h challenge compared with the shrimp in the control group (P<0 .05) .The expression level of glutathione peroxidase gene in the gills of the shrimp challenged with V . anguillarum was up‐regulated significantly to the peak 12 h after chal‐lenged (P<0 .05) ,then recovered to the level in the shrimp in the control group at 48 h .There was no significant changes in the expression level of catalase gene at first 6 h ,then up‐regulated at 12 h ,with the maximal level at 24 h (P<0 .05) .The same change tendency of superoxide dismutase and peroxide reduc‐tase gene expression were observed in the shrimp challenged with V .anguillarum ,increased to the peak at 3 h ,then kept a stable low level from 6 h to 72 h .The expression of glutathione S‐transferase gene was increased significantly to the peak level at 6 h ,and then decreased to the control group level gradually at 24 and 48 h .The findings indicated that V .anguillarum challenge had noticeable impact on antioxidant en‐zyme defense system in gills of the shrimp and the activities of antioxidant enzymes ,and the parameters measured in the present study were regarded as a monitor indicator for Vibrio diseases .
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Received: 25 November 2015
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