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| Effects of Probiotics on Body Composition ,Serum Biochemical Parameters ,Digestive Enzyme Activity and Intestinal Flora of Juvenile Jian Carp (Cy prinus carpio Var .Jian) |
| ZHANG Wen,ZHONG Lei,LI Nanchong,FANG Cheng,JI Hong ,REN Tongjun |
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Abstract A 80 day feeding trial was conducted in 3 ponds to investigate the effect of probiotics on body composition ,serum biochemical parameters ,digestive enzyme activity and intestinal flora in juvenile Jian carp (Cyprinus carpio Var .Jian) with initial body weight of (242 ± 9 .6) g .Jian carp juveniles were stocked into three 1334 m2 ponds without the probiotics (control group ,CG ) ,and with the probiotics (group Ⅰ ,ⅠG) and (group Ⅱ ,ⅡG) at stocking density of 20850 fish/hm2 ,and fed diet containing the probiotics inⅠG at feeding rate of 2% -4% three times a day .The water quality was monitored in the wa-ter periodically .The results showed that there was significantly lower crude fat in the hepatopancreas and serum activity of alanine aminotransferase (ALT) in the juveniles in ⅠG and ⅡG than those in CG(P<0.05) .There were significantly higher total protein and activities of amylase and lipase in ⅠG and ⅡG than those in CG (P<0 .05) .The alkaline protease in IG was significantly higher than in CG (P<0 .05) , and there was no difference in acid proteinase activity (P>0 .05) .There were 12 ,18 ,and 16 bands in the juvenles in CG ,Ⅰ G and Ⅱ G ,respectively ,with preponderant band .The similarity coefficients were 54.5% between ⅠG and probiotics ,55 .1% betweenⅡG and probiotics ,70 .2% between ⅠG and CG , 67.3% betweenⅡG and CG .The 13 bands by gel extraction ,cloning ,sequencing and alignment in NCBI revealed that the intestinal flora in Jian carp was primarily comprised of Bacilli , Spirochaetes ,β-Pro-teobacteria ,Clostridia ,andγ-Proteobacteria .It was concluded that the addition of probiotics can influence the metabolism of fish ,change the composition of body ,enhance the immunity and the activity of digestive enzyme to some extent ,and affect the composition of intestinal flora in jian carp ,which will promote the application of probiotics in aquaculture .
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Received: 25 December 2015
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