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| Composition of Heterotrophic Bacteria in a Recirculating Aquaculture System for Atlantic Salmon (Salmo sal ar) |
| WANG Xianping,LI Jie,HAN Houwei,HU Jiaping,ZHAO Lili,SHAO Wei,WANG Zhenyang |
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Abstract The aim of this work was to study the composition of heterotrophic bacteria in a Recirculating Aquaculture System(RAS)for Atlantic salmon (Salmo salar) and to compare the difference in bacterial isolation and cultivation in different culture media .The bacteria in water samples collected from the tanks were cultured in 6 types of medium in‐cluding 2216E ,BHI ,LB ,NA ,TSA ,and TCBS ,and composition and quantity of the bacteria were determined by a plate count method ,bacterial morphological identification and 16S rRNA gene sequencing analysis method .It was found that the heterotrophic bacteria in the tank water were divided into 4 major groups:Proteobacteria (including theα‐,γ‐Proteobacteria) ,Firmicutes ,Bacteroidetes ,and Actinobacteria ,with the dominant γ‐Proteobacteria ,especially the genera Pseudoal teromonas and Vibrio .The plate colony numbers in different media were shown as the order of 2216E >LB >TSA>BHI>NA> TCBS ,significantly higher in the 2216E than in the other 5 culture media (P<0 .01) .The bacteria isolated from 2216E culture medium belonged to 12 genera ,the maximal becteria in genus Pseud‐oalteromonas in accounting for 50% and genus Vibrio representing only 3 .13% .The genus Vibrio was the dominant genus in various culture media ,accounting for 33 .33% in BHI ,60% in LB ,25% in TSA and 100% in TCBS (only one genus V ibrio) ,but no V ibrio but Pseudomonas was isolated from NA culture mediuma .
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Received: 25 March 2016
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