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| Growth Trait and Genetic Diversity in the F1 Progeny of Dark Sleeper Odontobutis potamophila from Three Different Populations |
| WANG Peipei,DING Yandong,YU Xingda,JIA Xiuqi,ZHANG Guosong,YIN Shaowu,CHEN Shuqiao,ZHOU Guoqin |
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Abstract We chosed three different populations of dark sleeper Odontobutis potamop hila from Jiande , Dangtu and Taihu and mated by using 3 × 3 full diallel crossing .The growth traits and genetic diversity about the F1 offspring of nine combinations were compared and F1 progeny′s growth traits were evaluated in 2 months and 4 months .The length growth rate and the weight gain rate of the Jiande (♂) × Dangtu (♀) combination were shown to be 42 .58% and 190 .79% .The overall length heterosis ratio and the weight heterosis ratio of Jiande × Dangtu (cross and reciprocal cross ) combination were 17 .88% and 66 .75% ,higher than those in other combinations .There were higer length growth rate ,the weight gain rate and the heterosis rate in F1 progeny from different geographic populations than those in the same geo‐graphical population of the self‐reproducing F1 offspring .Subsequently ,the average value (0 .195) of hy‐brid progeny genetic differentiation index (Fst) was figured out by using eight microsatellite loci ,indica‐ting that there were genetic differences and the differentiation of nine combinations existed in offspring . The nine combinations of expected heterozygosity (He) were ranged from 0 .1493 to 0 .7971 and the ob‐served heterozygosity (Ho) ranged from 0 .1562 to 1 .0000 .The heterozygosity and the genetic diversity of hybrid progeny were higher than those of self‐reproducing progeny and the alleles (A) .The polymorphism information content (PIC) value of Jiande (♂) × Dangtu (♀) combination were 3 .9 and 0 .486 ,higher than other combinations .To synthesize the growth performance and the genetic diversity ,we suggest that determination of grow th and genetic diversity of the Jiande (♂) × Dangtu (♀ ) be the preliminary best combination of cross breeding .
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Received: 25 May 2016
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