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| Sequence Analysis of Mitochondrial Cytochrome b Gene in Four Sardinella Species |
| YANG Tian-yan,GAO Tian-xiang,GUO Yan,MENG Wei |
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Abstract The mitochondrial cytochrome b gene fragment (402 bp) of four Sardinella species were amplified and sequenced with the method of PCR, and the differences in the sequences were analyzed. Nine haplotypes were observed in ten samples. The average contents of T,C,A,and G were 28.9%,28.2%,23.9%,and 19.0%, respectively and the content of A+T was higher than that of G+C which was similar to the sequences of the other fishes. By the Kimura 2-parameter model, the genetic distances were found to change from 0.1616 to 0.2653. Based on sequences of partial cytochrome b gene, a phylogenetic tree by NJ method indicated that the Sardinella zunasi and S. jussieu, S. maderensis and S. aurita had a relatively close relationship, respectively. Molecular evolution rate showed that the divergence of four Sardinella species occurred during the middle and late Miocene about 800 to 1300 million years ago,indicating that the divergence time was earlier than the other fishes.
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Received: 25 November 2009
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