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| Optimization of Alginate-degrading Bacterial Fermentation Process for Alginate Lyase Production |
| HOU Bao-bing,LIU Shu-lai,ZHANG Jian-you,BAO Hua-fang,DING Yu-ting |
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Abstract The enzyme production of a bacterium strain with relatively high alginate lyase activity known as Acinetobacter X8 isolated from decaying part of seeweed Sargassum fusiform was investigated under liquid fermentation. The optimized liquid fermentation medium(m/V) was comprised of 0.6 % alginate, 0.7 % peptone, 0.2 % K_2HPO_4, and 2 % NaCl at initial pH of 7.0. After fermentation for 16 h at 150 r/min and 25 ℃, the yield of alginate lyase was found to be up to 157.32 U/ml, 159.01% higher than that under the ordinary conditions. However, the production process was inhibited by Mg~2, Ca~(2+) and Fe~(2+).
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Received: 25 November 2009
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