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| Construction and Analysis of Suppression Subtractive Hybridization Library of Dinoflagellate Alexandrium catenella |
| SHI Xi-zhi,LIU Bing,HE Jing-jing,WANG Meng-qian,GUO Hao,SU Xiu-rong,LI Tai-wu |
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Abstract To isolate the bioluminescence specific expressed genes of dinoflagellate Alexandrium catenella(A. catenella), suppression subtractive hybridization was performed to construct the differential expressing cDNA libraries of Alexandrium catenella. A total of 500 cDNA clones were picked. PCR amplification revealed that the length of inserted fragments was mainly from 250 to 1000 base pairs. Ten randomly selected clones after PCR screening were sequenced and analysed in GenBank with Blast search. Two ESTs shared significant identity with the luciferin binding protein and Ribulose-1,5-bisphosphate carboxylase/oxygenase and the other ESTs represent novel genes of Alexandrium catenella with no significant homology in GenBank. These results had provided the foundation for cloning new specific genes of Alexandrium catenella and further studying and establishing the novel detection method for specific dinoflagellata.
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Received: 25 November 2009
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