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| The Phylogenetic Relation of Sea Cucumber Acaudina molpadioidea |
| LI Hui,LI Tai-wu,SU Xiu-rong,QIN Yu-ming,WANG Meng-qian,HE Jing-jing,HU Guang-zhou,HUANG Shang-feng,ZHOU Jun |
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Abstract The phylogenetic relation of sea cucumber Acaudina molpadioidea from the coast in Zhejiang province were studied by analysis of 1312 bp from 18S rDNA and 692 bp from COImt DNA with homologous sequences of ten species within Holothuroidea downloaded from GenBank as well as the morphological characteristics. Molecular phylogenetic trees were constructed by Neighbor-Joining(NJ)and Maximum Parsimony(MP). The sea cucumber was found to be to Molpadiidae Holothuroidea, inferred from its Bio-Morphotyping. The 18S rDNA gene analysis reveals that the genetic distance between this sea cucumber and Pseudostichopus villosus is 0.017, that Acaudina molpadioidea belongs to Holothuroidea and is close to the Holothuroidea, Aspidochirota. Based on the COImt DNA gene analysis, there is close relationship between Acaudina molpadioidea and Pentamera lissoplaca, with long genetic distance of 0.223, not being the basis for the phylogenetic position.
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Received: 25 December 2009
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