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| Comparison of Embryonic Development in Gynogenetic and Diploid Barfin Flounder(Verasper moseri) |
| LI Zhong-hong,LIU Hai-jin,ZHANG Shi-kui,JIANG Xiu-feng,WANG YU-fen |
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Abstract Japanese flounder(Paralichthy olivaceus)sperm was used to induce haploid of gynogenetic barfin flounder(Verasper moseri), and homologous frozen sperm was used to induce normal barfin flounder embryos to test the methods of inducing gynogenesis in barfin flounder. Observation and comparison of the embryonic developments showed that the haploid embryonic development was slower than the normal embryo before multicelled stage and it lasted 14 hours. The early blastula of haploid embryonic development was found to be slower than the normal embryos while the late gastrula, neurula stage, and formation of eyelens stage were the same as the normal embryos. The haploid embryos had slower development rate than the normal embryos did, and the haploid syndrome was observed in the haploid larvae while the gynogenetic embryonic development had the similar character to the normal embryo. Lower hatching rate and higher malformation were found in the gynogenetic and haploid larvae compared with the normal embryos.
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Received: 25 December 2009
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