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| Cilico Cloning and Verification of eIF4A Gene from Laver Porphyra yezoensis |
| YI Le-fei,WANG Ping,ZHOU Xiang-hong |
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Abstract The silico cloning, verification and characterization of eukaryotic translation initiation factors 4 A(eIF4A)genes was conducted in cDNA of laver Porphyra yezoensis(PyeIF4A). The highly similar P. Yezoensis ESTs were obtained by Arabidopsis thaliana eIF4A amino acids sequence as a querying probe from dbEST of GenBank and the putative cDNA sequence of PyeIF4A was assembled. Furthermore, the cDNA of PyeIF4A was isolated by RT-PCR with two primers designed in accordance with the assembled sequence and was found to contain an 1278 nt of continuous complete open reading frame encoding a polypeptide(PyeIF4A)of 425 amino acids with a calculated molecular weight of 47.4 kDa. Sequence comparison of the PyeIF4A reveals high amino acid sequence identity with eIF4A from other organisms, such as mice Mus musculus, toad Xenopus laevis, rice Oryza sativa and plant Arabidopsis thaliana. The PyeIF4A contained conserved amino acids residue and sequences motifs closely related to function of eIF4A.
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Received: 25 December 2009
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