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| The Stress Effects of Temperature Fluctuation on Immune Factors in Crab Scylla serrata |
| DING Xiao-feng,YANG Yu-jiao,JIN Shan,WANG Guo-liang |
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Abstract The stress effects of temperature fluctuation on immune factors were studied in crab Scylla serrata. It was found that temperature changes have significant effect on the immune capacity of the cultured crab. There was the peak total hematocyte count(THC)at 20 ℃, and then gradual decline at higher or lower than 20 ℃, the minimum at 10 ℃ or 30 ℃. There was no significant difference in haemocyte phagocytosis percentage(PP)at all temperature, but the haemocyte phagocytosis index(PI)was very significant higher from 25 ℃ to 27 ℃ than at other temperture. Both lysozyme(LZM)and alkaline phosphatase(AKP)showed the maximal activities at 25 ℃, and declined as the temperature rose or decreased. The acid phosphatase(ACP), however, had the maximal activity at 20 ℃, and declined as the temperature increased or decreased. There were no significant differences in superoxide dismutase(SOD)and peroxidase(POD)(except for 30 ℃). The maximal phenol oxidase(PO)activity was observed at 30 ℃ and 27 ℃, while the PO activity showed no significant difference in other experimental groups. It can be inferred from the results that all the immunity factors in the crab had good performance when the temperature is varied from 20 ℃ to 25 ℃, and thus the crab has high capacity of disease resistance at this temperature.
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Received: 25 January 2010
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