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| The Optimal Culture Conditions of Bacterium Vibrio harveyi WG1702 from Clam Meretrix meretrix |
| LIU Lian-sheng,YAN Mao-cang,ZHAO Hai-quan,CHEN Qian-ying,MA Ai-min,LIN Zhi-hua |
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Abstract A bacterial strain WG1702( Vibrio harveyi) was isolated from diseased clam Meretrix meretrix during epidemic breakdown in Lvsi harbor,Jiangsu province,in September of 2007. The optimization of culture conditions showed that the strain WG1702 reached the peak growth in 32 h culture at temperature of 28 ℃,initial pH of 7,salinity of 40,and aeration ratio of 60 ml/150 ml. The metal ions such as NH_4~+,Fe~(2+) ,and Fe~(3+) were used as a cell growth enhancer,glucose as a carbon source and peptone as a nitrogen source. The orthogonal experiment for different age bacteria proved that the best growth was observed under the combination of the pH 7.11,temperature 28 ℃,and 24 h age bacteria. The variance analysis revealed that pH value had significantly influence on the growth of the bacterial strain.
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Received: 25 February 2010
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