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| Improving Enrichment Medium and Culture Conditions in Bacterium Vibrio parahaemolyticus |
| ZHAO Guang-ying,SHEN Ke-min,LI Jian-rong |
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Abstract The bacterium Vibrio parahaemolyticus enrichment medium and culture conditions were optimized by combination of two decisive factors peptone and yeast extract in the experiment designed by statistical software in order to obtain a method for enriching the bacterium. Direct analysis showed that the mixed peptone was found to be the major factor while the yeast extract was considered as the minor in the process of enrichment. The interaction between the two was ignored because of the weak performance. Variance analysis showed that F values of the mixed peptone and yeast extract were more than F_(0.05(1,1)), indicating that the whole values were at the significant level. The optimal medium formula selected by the orthogonal experimental design was described as the following:2% of the mixed peptone(1% tryptone, fish peptone 1%), 0.2% of yeast extract, 3.5% of NaCl,and pH 8.6. And the best results were observed under the culture conditions of (36 ±1) ℃, shaking table at 120 r/min and sealing with 2-layer kraft paper. In the same enrichment time, the concentration of enrichment broth of the bacterium by using the optimized medium and culture conditions was 1.8±0.6 times of the concentration of GB/T 4789.7-2008.
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Received: 25 March 2010
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