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| Rapid Detection of Pathogenic Bacteria of Ascites in Japanese Flounder(Paralichthys olivaceus)by Dot-ELISA |
| ZHU Zhuang-chun,CHEN Yang,HAO Dong-sheng,JIANG Guang-jian,XING Zhao-bin,WU Peng,BAI Chong-zhou,LI Jin-long,SHI Xiang-guo |
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Abstract A Dot-Enzyme Linked Immunosorbent Assay(Dot-ELISA)was successfully established for detection of Edwardsiella tarda found in Japanese flounder(Paralichthys olivaceus)by using nitrocellulose membrane as solid carrier. The working concentration of serum samples was 1∶800 dilution and that of the enzyme-labeled goat anti-rabbit IgG was 1∶200 dilution by chessboard assay. The samples with clear and obvious dots were judged as positive reaction. Edwardsiella tarda was positive in the Dot-ELISA, while Aeromonas sobria, A. hydrophila, Vibrio fluvialis, V.alginolyticus , V.anguillarum , Shewanella putrefaciens, Providencia alcalifaciens , Enterobacter sakazakii and Escherichia coli were negative in the assay. The trial indicated that this method was simple, specific, quick, direct, easy extention and application at basic units.
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Received: 25 March 2010
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