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| Heat Shock Transcription Factor 1 and Its Transcription Expression in Yesso Scallop Mizuhopecten yessoensis |
| WANG Jun,BAO Xiang-bo,JIANG Bing,LI Shi-lei,LIU Wei-dong |
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Abstract Heat shock transcription factors ,key factors to regulate the transcription of eukaryotic heat shock genes ,play important roles in heat shock response .In this study ,a full length of HSF1 (Heat shock transcription factor 1 ) cDNA was isolated from Yesso scallop Mizuhopecten yessoensis by homologous cloning and RACE method .The full-length was of 1944 bp ,encoding a polypeptide of 523 amino acids .Carried out multiple alignment and bioinformatics analysis ,the predicted amino acid sequence was found to share a conserved DNA-Binding domain ,HR-A /B and HR-C domain ,which showed highly similarities with other HSF1s .The expression of HSF1 mRNA in various tissues was examined using real-time quantitative RT-PCR (QRT-PCR) . HSF1 was expressed in all 6 tissues of the scallop ,and the mRNA expression was the maxinum in adductor muscle and the minimum in hemocytes .
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Received: 25 July 2013
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