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| Spermatogenous Analysis of Chinese Giant Salamander Males Which Are Induced to Spawn |
| QIAO Zhi-gang,LIU Xiao-cui,MA-Long,ZHANG Wei-fang,LI Yong-dong |
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Abstract Twenty Chinese giant salamander A ndrias davidianus males were artificially induced to spawn , with spawning success of 90% .However ,only 20% of the male spawners were found to release the vital sperm ,indicates that 80% of the male spawners were of sterile males . The vitality test showed that survival period of the sperm motility was different in the water with different hardness . The survival period of the sperm motility was found to be decreased with increase in water hardness within water hardness of 0 .17 ~ 2 .136 mmol/L .The salamander sperm showed the longest life span (161 s) at water hardness of 0 .41 mmol/L .On the contrary ,the minimal survival period (65 .4 s )was observed in the sperm at water hardness in the 3 .14 mmol/L .So we should pay attention to the problem of male sterile and effects of water hardness on fertility when we carry out artificial reproduction of Chinese giant salamander .
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Received: 25 July 2013
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