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| Effects of Industrial Effluent on Growth,Development and DNA in Tadpoles of Frog Rana nigromaculata |
| GAO Ai-bao,YUAN Li-xia |
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Abstract Tadpoles of frog Rana nigromaculata were reared in the water containing (in volume) 100% , 20% ,4% ,0 .8% and 0% of industrial effluent and average body weight ,body length and survival of the tadpoles were measured once every 7 days .The results showed that mortality rate was decreased from 3%in the control group to 100% in the 100% effluent group .Corresponding , the body length gain was descended from 43 .7% to 21 .1% ,at an average descended rate of 5 .6% with increase in the effluent volume at a rate of 5% during 14 days .The body weight gain was decreased from 238 .5% to 135 .6% ,at an average descended rate of 25 .7% with increase in the effluent volume at a rate of 5% during 14 days . The single cell gel electrophoresis (SCGE) was used to detect the breakage of DNA strand in hemocytes and the software of CASP was used to analyze the date picture .It was found that the DNA damage was shown to be of dose-effect and time-effect dependent .There was a significant increase in the levels of the damage of DNA ,as well as the tail length ,tail DNA ,and olive tail moment (OTM ) .The findings indicate that R .nigromaculata tadpoles can be used to monitor water pollution ,and that the present study is a foundation for the protection of amphibians and other aquatic organisms .
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Received: 25 July 2013
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