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| In Vitro Effect of Alcohol Extracts of 5 Traditional Chinese Medicines on Leukocyte Immune Activity in Peripheral Blood of Nile Tilapia |
| CHENG Min1,2,3, LI Zhongqin1,2,3, ZHAI Shaowei1,2,3, LAI Xiaojian1,2,3, YANG Qiuhua3,4, JIANG Xinglong1,2,3 |
1.Fisheries College, Jimei University, Xiamen 361021, China;
2.Eel Engineering Research Centre ofthe Modern Industry Technology, Ministry of Education, Xiamen 361021, China;
3.Engineering Technology ResearchCentre of Fishery Medicine in Xiamen, Xiamen 361021, China;
4.Fujian Fisheries Research Institute, Xiamen 361021, China |
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Abstract Chinese gall (Galla Chinensis), Chinese pulsaiilla root (Radix Pulsatillae), garden burnet root (Radix Sanguisorbae), fructus chebulae (Terminalia chebula), and giant knotweed rhizome (Rhizoma Polygoni Cuspidati) were powdered and sieved through a 160 mesh sieve, and then mixed with 60% aqueous solution of ethanol at the ratio of material to liquid 1∶10,finally ultrasonicated by an ultrasonic pulverizer for 1 hour. The filtrate was collected by filtration through a filter paper, and concentrated in a multi-sample parallel evaporator to obtain a Chinese medicine extract. About 0.5 mL of blood was collected from the caudal vein of Nile tilapia (Oreochromis niloticus) using a sterile syringe with 0.2 mL of anticoagulant, and mixed with L-15 cell medium at a ratio of 1∶9 by volume to form plasma. The Chinese herbal medicine extract was incubated with the Chinese herbal medicine extract alone and in combination and the effects of Chinese herbal medicine extract on the respiratory burst activity and leukocyte phagocytic activity in peripheral blood leukocytes of Nile tilapia were determined by nitrogen blue tetrazolium reduction method and flow cytometry. Single use of Chinese gall, Chinese pulsaiilla root, garden burnet root, fructus chebulae, and giant knotweed rhizome was shown to lead to significantly enhance the leukocyte oxygenation burst activity by 128.68%, 123.96%, 28.37%, 44.44%, and 78.93%, and the phagocytic rate elevate by 44.04%, 44.66%, 95.00%, 10.35% and 64.93%, respectively. In the double-use experiment of two traditional Chinese medicines, the combination showing synergistic effect on the leukocyte oxygen respiratory burst activity was Chinese pulsaiilla root + garden burnet root, with the combined respiratory burst index of 1.28. There were 4 combinations showing synergistic effects on phagocytic activity of leukocyte, including Chinese pulsaiilla root + giant knotweed rhizome, fructus chebulae + giant knotweed rhizome, Chinese gall + giant knotweed rhizome, and Chinese pulsaiilla root + fructus chebulae, with the phagocytic combined index of 11.45, 6.20, 3.03, and 1.06, respectively.
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Received: 14 January 2019
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