急性升温胁迫与恢复对葛氏鲈塘鳢抗氧化酶活性的影响

doi:10.16378/j.cnki.1003-1111.2020.03.012

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (9630 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Amur sleeper Perccottus glenii with body weight of (13.41±6.31) g was divided into three groups and reared in a glass aquarium of 80 cm×60 cm×40 cm at water temperature of 17 ℃ (control group), gradual warming at a rate of 1 ℃/d (gradual warming group), and in acute warming group, the test fish being transferred directly from 17 ℃ aquarium to 31 ℃ aquarium. In the two groups the water temperature was kept at constant 31 ℃ and the activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) and malondialdehyde (MDA) content were determined in liver, brain and muscle of Amur sleeper to understand the effects of acute warming stress on the antioxidant capacity and mortality rate of Amur sleeper and to provide references with industrial aquaculture of Amur sleeper. The results showed that there were significantly higher mortality rate and activities of antioxidant enzymes in liver and brain in the Amur sleeper in the acute warming group than those in the gradual warming group (P<0.05). The activities of SOD and GPX in brain were found to be significantly higher in the Amur sleeper in the acute warming group than those in gradually warming group after 6 hours (P<0.05). The activities of SOD and GPX, however, were significantly lower than those in the control group during the recovery to 17 ℃ (P<0.05), and the activities of SOD in muscle were significantly higher than those in gradually warming group at 12 hours (P<0.05). The content of MDA in liver was significantly higher in the Amur sleeper in the acute warming group than that in control group within 1 hour (P<0.05). The content of MDA in the brain was significantly higher than that in the control group and the gradually warming group (P<0.05), and in the muscle was significantly higher than that in the gradually warming group (P<0.05), while the content of MDA in the brain during recovery was significantly higher than that in the control group (P<0.05). The findings indicated that liver and brain injury was not recovered under long-term high temperature stress, and muscle was more adaptable to acute warming than liver and brain. GPX in the brain first responded to oxidative damage caused by acute warming, and then increases in SOD and CAT activities inhibitting GPX activity. Amur sleeper can survive longer under the condition of gradual warming than under acute warming.

Key words： Amur sleeper Perccottus glenii acute warming antioxidant enzyme

Received: 09 April 2019

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WANG Boya
	GUO Ce
	HUANG Puyi
	CHAI Longhui

Cite this article:

WANG Boya,GUO Ce,HUANG Puyi, et al. Effects of Acute Raising Temperature Stress and Recovery on Antioxidant Enzyme Activities of Amur Sleeper Perccottus glenii[J]. Fisheries Science, 2020, 39(3): 394-399.

URL:

https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.2020.03.012 OR https://www.shchkx.com/EN/Y2020/V39/I3/394

[1]曲丽华,管红梅,巩丽娟. 北方地区养殖葛氏鲈塘鳢前景展望[J]. 黑龙江水产,2018(3):12-14.
[2]刘义新,金广海,杨培民. 葛氏鲈塘鳢工厂化人工繁殖技术研究[J]. 农技服务,2016,33(13):139.
[3]刘义新,金广海,杨培民,等. 3种常用水产药品对葛氏鲈塘鳢的急性毒性研究[J]. 江西水产科技,2017,(1):10-12.
[4]Lu Y, Wu Z, Song Z, et al. Insight into the heat resistance of fish via blood: effects of heat stress on metabolism, oxidative stress and antioxidant response of olive flounder Paralichthys olivaceus and turbot Scophthalmus maximus[J]. Fish & Shellfish Immunology,2016,58:125-135.
[5]宋志明,刘鉴毅,庄平,等. 低温胁迫对点篮子鱼幼鱼肝脏抗氧化酶活性及丙二醛含量的影响[J]. 海洋渔业,2015,37(2):142-150.
[6]李文龙,梁兴明,梁萌青,等. 温度对大菱鲆幼鱼生长及免疫相关酶活性的影响[J]. 水产科学,2017,36(3):311-316.
[7]许治冲,刘晖,徐奇友,等. 温度和饲料脂肪水平对松浦镜鲤免疫及抗氧化能力的影响[J]. 大连海洋大学学报,2012,27(5):429-435.
[8]徐冬冬,楼宝,詹炜,等. 高温胁迫对褐牙鲆生长及肝脏抗氧化酶活性的影响[J]. 水产学报,2010,34(7):1099-1105.
[9]刘峰,刘阳阳,楼宝,等. 温度对小黄鱼体内抗氧化酶及消化酶活性的影响[J]. 海洋学报,2016,38(12):76-85.
[10]史鲲鹏,董双林,周演根,等.不同倍性虹鳟幼鱼对急性温度胁迫的抗氧化响应[J].应用生态学报,2018,29(9):3102-3110.
[11]邵彦翔, 陈超, 张廷廷, 等. 高温胁迫对2种杂交石斑鱼存活率及血清生化指标的影响[J]. 广东海洋大学学报,2017,37(6):89-95.
[12]强俊,任洪涛,徐跑,等. 温度与盐度对吉富品系尼罗罗非鱼幼鱼生长和肝脏抗氧化酶活力的协同影响[J]. 应用生态学报,2012,23(1):255-263.
[13]Yang S, Yan T, Zhao L, et al. Effects of temperature on activities of antioxidant enzymes and Na⁺/K⁺-ATPase, and hormone levels in Schizothorax prenanti[J]. Journal of Thermal Biology,2018,72:155-160.
[14]Madeira D, Narciso L, Cabral H N, et al. Influence of temperature in thermal and oxidative stress responses in estuarine sh[J]. Comparative Biochemistry and Physiology,Part A,2016,166(2):237-243.
[15]Tincy V, Kumar P A, Putyottil M, et al. Physiological and molecular responses of a bottom dwelling carp, Cirrhinus mrigala to short-term environmental hypoxia[J]. Turkish Journal of Fisheries and Aquatic Sciences,2017,18(3):483-490.
[16]刘汝建,区又君,李加儿,等. 盐度、温度对卵形鲳鲹选育群体肝抗氧化酶活力的影响[J]. 动物学杂志,2013,48(3):428-436.
[17]刘超,曹金凤,马甡.条纹锯鮨对高温胁迫及胁迫后恢复方式适应的初步研究[J]. 海洋科学,2011,36(9):54-58.
[18]潘桂平,刘本伟,周文玉. 低温胁迫对云纹石斑鱼幼鱼抗氧化和免疫指标的影响[J]. 上海海洋大学学报,2016,25(1):78-85.
[19]Joy S, Alikunju A P, Jose J, et al. Oxidative stress and antioxidant defense responses of Etroplus suratensis to acute temperature fluctuations[J]. Journal of Thermal Biology,2017,70(Pt B):20-26.
[20]Liu Y, Ma D, Zhao C, et al. Histological and enzymatic responses of Japanese flounder (Paralichthys olivaceus) and its hybrids (P. olivaceus♀× P. dentatus♂) to chronic heat stress[J]. Fish Physiol Biochem,2014,40(4):1031-1041.
[21]Madeiraa D, Vinagre C, Diniz M S. Are fish in hot water? Effects of warming on oxidative stress metabolism in the commercial species Sparus aurata[J]. Ecological Indicators,2016,63:324-331.