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| Microscopic Observation of Chromatophores in Skin, Scale, and Fin of Mandarinfish Siniperca chuatsi |
| WU Minglin1,2, CHEN Xiaowu3, CUI Kai1,2, LI Haiyang1,2, HOU Guanjun1,2, JIANG Yangyang1,2 |
1. Fisheries Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China;
2. Anhui Province Key Laboratory of Aquaculture & Stock Enhancement, Hefei 230031, China;
3. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China |
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Abstract The types, distribution, and morphological characteristics of chromatophores were histologically observed in skin, scale, and fin of 1 year old mandarinfish Siniperca chuatsi with body weight of 500 g by frozen section and routine HE staining section under a light microscope. Longitudinal sections of skin showed that there were primarily melanophores in the head posterior to eye, the black spot at body side, and the lower part at body side. The throat and abdomen mainly contained iridophores. Transverse microscopic observation of skin showed that the melanophores on the surface of skin were aggregated into a mass or a lump, with granular xanthophores, and long fusiform iridophores. Under HE staining, the melanophores in skin were brown and black in color and lumpy in shape, and the iridophores were light gray in color. The skin pigment layers were mainly distributed between the epidermis layer and the dermis loose layer, on the dermis dense layer connected with the muscle, with a small number of chromatophores distributed in the fibrous tissue of the dermis loose layer. In scales, the melanophores and xanthophores were mainly found in the head posterior to eye, the black spot at body side, and the lower part at body side. No or a small number of melanophores and xanthophores were observed in the transparent throat and abdomen. The melanophores on scale had a large cell body containing many thin branches, and the xanthophores were granular. There were melanophores and xanthophores in fins, with significant differences in the proportion, size, and coloration in different parts. In the long-term evolution process, the stripes and patterns with unique characteristics of mandarinfish are formed by the different blending ratios and colorations of melanophores, xanthophores, and iridophores, which are conducive to hiding and predation, and have a certain degree of ornamental value.
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Received: 18 July 2019
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