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| Structural Analysis of Intestinal Microflora in Wild and Cultured Schizothorax o'connori |
| WANG Jinlin, WANG Qielu, WANG Wanliang, ZENG Benhe, PAN Yingzi, ZHOU Jianshe |
| Institute of Fisheries Science, Xizang Autonomous Region Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850002, China |
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Abstract MiSeq high-throughput sequencing technology and bioinformatics analysis were used to construct gene sequencing libraries of intestinal microflora structure in six samples in wild and cultured Schizothorax o'connori with body weight of about 1 kg to compare diversity differences in structural and biological richness in intestinal microflora between wild and cultured S. o'connori. The results showed that the OTUs data of wild group samples Y1, Y2, Y3 and domesticated group samples C1, C2 and C3 were divided into 751, 469, 313, and 302, 259, 263, respectively, without significant difference in the richness and diversity of intestinal flora between wild and cultured animals (P>0.05). Actinobacteria and Proteobacteria were the dominant flora in the wild fish, while Cyanobacteria and Proteobacteria were the dominant flora in the cultured fish. Micrococcus and Aeromonas were the main taxonomic bacteria in the wild group, while Cyanobacteria were the main taxonomic bacteria in the cultured group. The findings provide a theoretical basis for the healthy culture of S. o'connori and the construction of micro-ecological control technology of culture environment.
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Received: 10 May 2019
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