|
|
|
| Acute Toxicity of Four Drugs Used in Aquaculture to Juvenile Leech Whitmania pigra |
| GUO Kun1,2, LUO Mingzhong1,2, RUAN Guoliang1, WEI Wei2, LI Rui2 |
1. Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou 434025, China;
2. State Key Laboratory for Quality and Safety of Agroproducts (in Preparation), |
|
|
|
|
Abstract Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China Juvenile leech Whitmania pigra with body weight of (0.062±0.006) g was placed in an 80 mesh bag of 10 cm×10 cm×5 cm fixed and submerged in a 40 cm×50 cm×30 cm plastic tank, and the acute toxicity of copper sulfate, povidone iodine, potassium permanganate and phoxim to juvenile leech was studied under water temperature of (25.0±1.5) ℃, pH 7.2—7.8 and dissolved oxygen level (DO) of (6.0±0.5) mg/L by a semi-static test method. The results showed that the 24 h median lethal concentration (LC50) was found to be 0.579 mg/L, with safe concentration of 0.053 mg/L for copper sulfate; 78.173 mg/L, with safe concentration of 15.367 mg/L, for povidone iodine; 3.956 mg/L, with safe concentration of 0.706 mg/L for potassium permanganate and 0.978 mg/L, with safe concentration of 0.183 mg/L for phoxim, the 48 h LC50 0.389 mg/L for copper sulfate, 67.898 mg/L for povidone iodine, 3.327 mg/L for potassium permanganate and 0.836 mg/L for phoxim; the 72 h LC50 0.280 mg/L for copper sulfate, 62.747 mg/L for povidone iodine, 2.703 mg/L for potassium permanganate and 0.611 mg/L for phoxim, and the 96 h LC50 0.241 mg/L for copper sulfate, 56.058 mg/L for povidone iodine, 2.313 mg/L for potassium permanganate and 0.493 mg/L for phoxim. According to the toxic classification standards published by state environmental protection administration, copper sulfate and phoxim were within the high toxic substances,potassium permanganate was within the medium toxic substances, and povidone iodine belonged to the low toxic substances for juvenile leech.
|
|
Received: 10 May 2019
|
|
|
|
|
|
[1]国家药典委员会.中华人民共和国药典:一部 [S].北京:中国医药科技出版社,2015.
[2]Liu F, Shi H Z, Guo Q S, et al.Isolation and characterization of microsatellite loci for the analysis of genetic diversity in Whitmania pigra [J].Biochemical Systematics and Ecology,2013,51:207-214.
[3]Wang Y, Zhao X, Wang Y S, et al.An extract from medical leech improves the function of endothelial cells in vitro and attenuates atherosclerosis in ApoE null mice by reducing macrophages in the lesions [J].Biochemical and Biophysical Research Communications,2014,455(1/2):119-125.
[4]刘飞,杨大坚.中国水蛭人工养殖的现行模式调研 [J].世界科学技术—中医药现代化,2014,16(10):2170-2173.
[5]史红专,刘飞,郭巧生.温度对蚂蟥生长及摄食规律影响的初步研究 [J].中国中药杂志,2006,31(23):1944-1946.
[6]熊良伟,王帅兵,王建国,等.宽体金线蛭繁殖性能及蛭苗生长特征研究 [J].上海海洋大学学报,2016,25(3):374-380.
[7]郭坤,罗鸣钟,苏应兵,等.土壤湿度及稻草基质对宽体金线蛭繁殖性能的影响[J].南方农业学报,2017,48(10):1918-1922.
[8]王信海,姜爱兰,唐金玉,等.不同地理种群宽体金线蛭繁殖力及其初孵稚蛭、幼蛭成活率和生长指标比较[J].大连海洋大学学报,2018,33(5):576-582.
[9]吴雷明,王守红,张家宏,等. 不同孵化基质和开口饵料对宽体金线蛭生长发育的影响[J]. 水产科学,2019,38(2):260-265.
[10]王建国,熊良伟,陶桂庆,等.不同体质量宽体金线蛭对饵料螺的摄食规律及生长特性 [J].江苏农业科学,2018,46(18):168-171.
[11]王建国,熊良伟,陶桂庆,等.宽体金线蛭苗对萝卜螺和方形环棱螺仔螺摄食及生长特征研究 [J].中药材,2018,41(5):1022-1026.
[12]Wang J, Guo Q S, Shi H Z, et al.Effects of light spectrum and intensity on growth, survival and physiology of leech (Whitmania pigra) larvae under the rearing conditions [J].Aquaculture Research,2017,48(7):3329-3339.
[13]张莺莺,谈永松,曹建国,等. 两种养殖密度下稻田宽体金线蛭生产效益分析[J]. 水产科学,2019,38(6):856-861.
[14]牟洪民,唐黎,王吉桥,等.4种常用渔药对鲢鱼种的急性毒性试验 [J].淡水渔业,2010,40(5):76-79.
[15]周永欣,章宗涉.水生生物毒性试验方法 [M].北京:农业出版社,1989:79-83.
[16]罗鸣钟,杨曼绮,郭坤,等.4种有机磷农药对黄鳝幼鱼的急性毒性研究 [J].淡水渔业,2018,48(5):66-72.
[17]蔡道基.农药环境毒理学研究 [M].北京:中国环境科学出版社,1999:128-134.
[18]国家环保局《水生生物监测手册》编委会.水生生物监测手册 [M].南京:东南大学出版社,1993:47.
[19]刘祖毅,王志铮,吕敢堂,等.3种有机磷农药对黑鲷幼鱼的急性毒性研究 [J].浙江海洋学院学报:自然科学版,2010,29(1):20-24.
[20]张涛,于翔,龚元,等.四种常用水产药物对菲牛蛭急性毒性的研究 [J].水产学杂志,2014,27(3):52-56.
[21]Cerqueira C C C, Fernandes M N.Gill tissue recovery after copper exposure and blood parameter responses in the tropical fish Prochilodus scrofa [J]. Ecotoxicol Environ Safety,2002,52(2):83-91.
[22]吕豪,梁峻,龙强.聚维酮碘对海湾扇贝等贝类幼虫的毒性试验研究[J].水产科学,2003,22(6):8-11.
[23]Zamora J L.Chemical and microbiologic characteristic and toxicity of povidone-iodine solutions [J]. The American Journal of Surgery,1986,153(3):400-406.
[24]农业部《新编渔药手册》编撰委员会.新编渔药手册[M].北京:中国农业出版社,2005:187-189.
[25]韩小丽,高攀,胡建勇,等.七种常用水产药物对梭鲈幼鱼的急性毒性 [J].水产学杂志,2015,28(3):26-29.
[26]Altuntas I, Delibas N, Doguc D K, et al.Role of reactive oxygen species in organophosphate insecticide phosalone toxicity in erythrocytes in vitro [J].Toxicology in Vitro,2003,17(2):153-157. |
|
|
|
