超高效液相色谱—串联质谱法测定鱼、虾中4种抗病毒药物的残留

doi:10.16378/j.cnki.1003-1111.2020.04.021

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Abstract An efficient ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for the simultaneous analysis of residues of 4 antiviral drugs ( amantadine, rimantadine, morpholine, and memantine) in fish and shrimp. Samples were extracted with 1% trichloroacetic acid and 1% acetic acid acetonitrile (1∶9, V/V), purified by PSA powder, C18 powder, neutral alumina dispersed solid phase extraction. Quantifification of the analytes was achieved by UPLC-MS/MS using the internal standard method. The limit of detection (LOD) and limit of quantifification (LOQ) of the method were lower than 0.8 μg/kg and 1.0 μg/kg for all the antiviral drugs, respectively. The average recoveries of 4 antiviral drugs in blank fish and shrimp at spiked levels of 1.0, 10.0, and 50.0 μg/kg ranged from 86.0% to 98.5%, with relative standard deviation (RSD) of less than 8%. The method is featured by small matrix interference, high sensitivity and good recovery rate, and can be used for residue detection of antiviral drugs in daily fish and shrimp samples.

Key words： ultra high performance liquid chromatography-tandem mass spectrometry dispersive solid phase extraction antiviral drug residue fish shrimp

Received: 29 July 2019

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	Articles by authors
	ZHANG Yukun
	LIU Zhenxian
	XU Ge
	ZHENG Xiaoying
	LIU Jinxin
	MIAO Xiaoqun

Cite this article:

ZHANG Yukun,LIU Zhenxian,XU Ge, et al. Determination of Residues of Four Antiviral Drugs in Fish and Shrimp by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Fisheries Science, 2020, 39(4): 609-614.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.2020.04.021 OR https://www.shchkx.com/EN/Y2020/V39/I4/609

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