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| Effect of Flowing on Opening Culture of Diatom Nitzschia closterium f. minutissima |
| LENG Xiaofei1, ZHANG Bo2, CHENG Dejin1, ZHOU Mi3, QI Shoubing4, CHEN Shun3, ZHANG Weijie3 |
1. Dalian Haibao Fishery, Co., Ltd., Dalian 116041, China;
2. Marine and Fishery Supervision and Supervision Brigade of Rushan City, Weihai 264500, China;
3. Key Laboratory of Mariculture & Stock Enhancement in the North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China;
4. School of Marine Resources and Environment, Jiangsu Ocean University, Lianyungang 210095, China |
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Abstract Twelve 200 L (53 cm×72 cm×58 cm) water tanks with diatom Nitzschia closterium f. minutissima at initial density of 7.0×104 cells/mL were divided into 4 groups, and 3 W (group 1), 6 W (group 2), or 12 W(group 3) flow pumps were disposed at 20 cm from the bottom of the long surface of the tank with flow rate of 2000 L/h, 3000 L/h, and 5000 L/h with the water flow angle of 45° toward the bottom of the tank, the fourth tank (group 4) with manual agitation as control group, under natural light for 6 d to investigate the effect of different power flowing on the open culture efficiency of the diatom. The results showed that there was significantly higher microalga cell density in the three flow making groups than that in the control group (P<0.05) from the 3rd day to the end of the experiment, with (116.7±4.5)×104 cells/mL in group 1, (133.8±4.0)×104 cells/mL in group 2, and (144.5±3.8)×104 cells/mL in group 3, and 53.1%, 74.6%, and 93.1% higher than that in the control group [(76.3±4.7)×104 cells/mL], significant difference among the three flow making groups (P<0.05), significantly higher than that in the control group (P<0.01). No small blockings were observed in microalga cell in making flowing groups during the diatom culture, with significantly higher temperature in group 2 and group 3 at several time (P<0.05). There was significantly higher pH value in the three flowing groups than that in the control group after the noon of the 5th day (P<0.05). Correlation analysis revealed that the correlation coefficient (R) was 0.923 between the medium pH and microalga cell density in the control group, and were ranged from 0.960 to 0.975 in the flowing groups. The regression models to predict cell density by pH was established via regression analysis, with higher determinate coefficients (r2) of the models in the flowing groups (0.9525—0.9685) than that in the control group(0.8588).
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Received: 05 June 2019
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