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| Genetic Diversity of Goby Rhinogobius giurinus in the Fragmented Habitat in Nanpan River |
| DING Xuemei1,2, YAN Yuehui1,2, LI Qiang2, LUO Xu1, TANG Lizhou2 |
1. Key Laboratory for Conserving Wildlife with Small Populations in Yunnan Province, Southwest Forestry University, Kunming 650224, China;
2. Center for Yunnan Plateau Biological Resources Protection and Utilization, Qujing Normal University, Qujing 655011, China |
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Abstract Multiple water conservancy facilities and the associated lakes result in fragmented habitats in Nanpan River areas. We investigated the effect of fragmented habitats on the population genetic diversity of goby Rhinogobius giurinus. Since the goby has never been domesticated because of low economic value, this species is suitable for evaluating the effect of fragmented habitats. Mitochondrial COXⅠgene and Cyt b gene were used as molecular markers for the analysis of the genetic diversity and differentiation of goby populations from 13 plateau lakes of the Nanpan River. In the COXⅠgene, Cytb gene and combined mitochondrial DNA sequences, 27, 22, 49 variable sites and 11, 13, 26 haplotypes were observed among 194 individuals belonging to 13 goby populations, with average haplotype diversity of 0.691, 0.813, 0.881 and nucleotide diversity of 0.0053, 0.0060, 0.0056. The phylogenetic trees showed that 13 populations of goby were divided into two branches without obvious phylogeographic pattern. The analysis of molecular variance (AMOVA) showed that the genetic differentiation mainly occurred among populations. Overall, it was found that the goby in the fragmented habitats of Nanpan River had high genetic diversity as well as obvious genetic differentiation. Probably due to strong adaptability and stable effective population size, the goby displayed a high level of genetic diversity. Besides, both weak migration ability and fragmented habitats led to significant genetic differentiation of the goby.
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Received: 12 September 2019
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