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| Morphological Description and Phylogeny of Tilapia Parasitic Cichlidogyrus |
| RONG Mengjie, KADIR Kadirdir, ZHANG Wenrun, YUE Cheng, HAO Cuilan |
| College of Animal Medicine, Xinjiang Agricultural University, rümqi 830052, China |
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Abstract From November 2018 to May 2019, during the investigation of fish parasites in the markets and farms around rümqi City, it was found that there were a large number of Ancyrocephalidae in the gills of tilapia in different regions. After dye observation,the structure of the copulatory organs and haptors were mapped, measured and compared. At the same time, three molecular markers (28S, 18S-ITS1-5.8S and COⅠ) were selected for amplification, cloning and sequencing and phylogenetic analysis was carried out with ribosomal RNA and related species. The results showed that the morphological characteristics preliminarily identified the anchored worm as Ancyrocephalidae, Cichlidogyrus sclerosus (MJ 12) and C. mbirizei (MJ 23 or MJ 33) in Cichlidogyrus. The 28S, 18S-ITS1-5.8S and COⅠ sequence fragment size of MJ12 were 863 bp, 1019 bp and 592 bp, respectively, the similarities with C. sclerosus published in Genbank were above 99.5%; the 28S, 18S-ITS1-5.8S and COⅠsequences of MJ23 were 863 bp, 1005 bp and 572 bp, respectively, the similarity between MJ23 and C. mbirizei in GenBank was over 94%, and the similarity between MJ23 and C. cirratus was over 99.3%. According to the ML tree and genetic matrix constructed based on ribosomal RNA, MJ12 and Chitin cichlids from different regions are clustered together, and the genetic distance was 0; the topological structure formed by MJ 23 or MJ 33 and the C. mbirizei in Brazil and the C. cirratus had a self-expanding value of 99% and 100%, with genetic distance of 0.000—0.003, consistent with the morphological result.The findings supplemented the domestic molecular data of the worm, and also supported the viewpoint of the classification of synonymy species of C. mbirizei and C. cirratus.
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Received: 09 December 2019
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[1]陈泰芝,叶卫. 我国引进的罗非鱼类的初步研究[J]. 动物学杂志,1994,29(3):18-23.
[2]Xiong W, Sui X Y, Liang S H, et al. Non-native freshwater fish species in China[J]. Reviews in Fish Biology and Fisheries,2015,25(4):651-687.
[3]张永德,林勇,潘传燕,等.尼罗罗非鱼MyD88基因的表达及多克隆抗体制备[J].水产科学,2019,38(5):595-602.
[4]Pariselle A, Euzet L. Systematic revision of dactylogyridean parasites (Monogenea) from cichlid fishes in Africa, the Levant and Madagascar[J]. Zoosystema,2009,31(4):849-898.
[5]Vanhove M P M, Volckaert F A M, Pariselle A. Ancyrocephalidae (Monogenea) of Lake Tanganyika: Ⅰ: four new species of Cichlidogyrus from Ophthalmotilapia ventralis (Teleostei: Cichlidae), the first record of this parasite family in the basin[J]. Zoologia (Curtiba),2011,28(2):253-263.
[6]Gillardin C, Vanhove M P M, Pariselle A, et al. Ancyrocephalidae (Monogenea) of Lake Tanganyika: Ⅱ: description of the first Cichlidogyrus spp. parasites from Tropheini fish hosts (Teleostei, Cichlidae)[J]. Parasitology Research,2012,110(1):305-313.
[7]Muterezi Bukinga F, Vanhove M P M, Van Steenberge M, et al. Ancyrocephalidae (Monogenea) of Lake Tanganyika: Ⅲ:Cichlidogyrus infecting the world′s biggest cichlid and the non-endemic tribes Haplochromini, Oreochromini and Tylochromini (Teleostei, Cichlidae)[J]. Parasitology Research,2012,111(5):2049-2061.
[8]Pariselle A, Bitja Nyom A R, Bilong C F. Checklist of the ancyrocephalids (Monogenea) parasitizing tilapia species in Cameroon, with the description of three new species[J]. Zootaxa,2013,3599:78-86.
[9]Ibrahim M M.Variation in parasite infracommunies of Tilapia zillii in relation to some biotic and abiotic factors[J]. International Journal of Zoological Research,2012,8(2):59-70.
[10]$\check{R}$ehulkov E, Mendlov M, imkov A. Two new species of Cichlidogyrus (Monogenea:Dactylogyridae) parasitizing the gills of African cichlid fishes (Perciformes) from Senegal:morphometric and molecular characterization[J]. Parasitology Research,2013,112(4):1399-1410.
[11]吴相云,谢明权,李安兴.奥尼罗非鱼寄生单殖吸虫中国二新纪录属(锚首虫科)[J].动物分类学报,2006,31(3):536-540.
[12]李海燕,陈文婷,满国波,等.珠江水系外来鱼类单殖吸虫研究Ⅰ——尼罗罗非鱼鳃上4种单殖吸虫(包括3新纪录种)的描述[J].广州大学学报(自然科学版),2009,8(3):54-57.
[13]孟飞燕.澜沧江景洪流域10种鱼类单殖吸虫的形态学分类及双身虫科和锚首虫科的分子鉴定[D].昆明:云南师范大学,2016.
[14]白俊萍.澜沧江9种鱼类单殖吸虫的形态分类鉴定及基于ITS-2序列双身虫科的系统发育研究[D].昆明:云南师范大学,2013.
[15]肖靖芳.澜沧江11种鱼类寄生指环虫科和锚首虫科单殖吸虫的形态学和宿主特异性研究[D].昆明:云南师范大学,2015.
[16]Zhang S, Zhi T T, Xu X L, et al. Monogenean fauna of alien tilapias (Cichlidae) in south China[J]. Parasite,2019,26(4):16.
[17]丁雪娟,廖翔华,李安兴,等.单殖吸虫分子系统学研究进展[J].寄生虫与医学昆虫学报,2005,12(2):112-118.
[18]吴相云,谢明权,李安兴.基于28S rDNA C1~D2区的鳞盘虫科单殖吸虫系统学初步研究[J].动物分类学报,2007,32(3):593-598.
[19]Pariselle A, Euzet L. Gill parasites of the genus Cichlidogyrus Paperna,1960 (Monogenea, Ancyrocephalidae) from Tilapia guineensis (Bleeker, 1862),with descriptions of six new species[J].Systematic Parasitology,1995,30(3):187-198.
[20]Hassouna N, Michot B, Bachellerie J P. The complete nucleotide sequence of mouse 28S rRNA gene. Implications for the process of size increase of the large subunit rRNA in higher eukaryotes[J]. Nucleic Acids Research,1984,12(8):3563-3583.
[21]imkov A, Plaisance L, Matějusov I, et al. Phylogenetic relationships of the Dactylogyridae Bychowsky, 1933 (Monogenea: Dactylogyridea): the need for the systematic revision of the Ancyrocephalinae Bychowsky,1937[J]. Systematic Parasitology,2003,54(1):1-11.
[22]Vanhove M P M, Pariselle A, Van Steenberge M, et al.Hidden biodiversity in an ancient lake: phylogenetic congruence between Lake Tanganyika tropheine cichlids and their monogenean flatworm parasites[J].Scientific Reports,2015,5:13669.
[23]Doullou L. Monogeneans of the genus Cichlidogyrus Paperna, 1960 (Dactylogyridae: Ancyrocephalinae) from cichlid fishes of Lake Kariba (Zimbabwe) with descriptions of five new species[J].Systematic Parasitology,1993,25(3):159-186.
[24]Rindoria N M, Mungai L K, Yasindi A W, et al. Gill monogeneans of Oreochromis niloticus (Linnaeus, 1758) and Oreochromis leucostictus (Trewavas,1933) in Lake Naivasha, Kenya[J].Parasitology Research,2016,115(4):1501-1508.
[25]Akoll P, Fioravanti M L, Konecny R, et al. Infection dynamics of Cichlidogyrus tilapiae and C. sclerosus (Monogenea, Ancyrocephalinae) in Nile tilapia (Oreochromis niloticus L.) from Uganda[J].Journal of Helminthology,2012,86(3):302-310.
[26]Maneepitaksanti W, Nagasawa K. Monogeneans of Cichlidogyrus Paperna, 1960 (Dactylogyridae), gill parasites of tilapias, from Okinawa Prefecture, Japan[J].Biogeography,2012,14(2):111-119.
[27]Mendoza-Franco E F, Caspeta-Mandujano J M, Tapia Osorio M T. Ecto- and endo-parasitic monogeneans (Platyhelminthes) on cultured freshwater exotic fish species in the state of Morelos, South-Central Mexico[J]. ZooKeys,2018,776:1-12.
[28]Paperna I, Thurston J P. Monogenetic trematodes collected from cichlid fish in Uganda; including the description of five new species of Cichlidogyrus[J].Revue De Zoologie Et De Botanique Africaines,1969,13(1):76-78.
[29]Lerssutthichawal T, Maneepitaksanti W, Purivirojkul W. Gill Monogeneans of potentially cultured tilapias and first record of Cichlidogyrus mbirizei Bukinga et al,2012, in Thailand[J]. Walailak Journal of Science and Technology,2016,13(7):543-553.
[30]Vignon M, Pariselle A, Vanhove M P M. Modularity in attachment organs of African Cichlidogyrus (Platyhelminthes:Monogenea:Ancyrocephalidae) reflects phylogeny rather than host specificity or geographic distribution[J]. Biological Journal of the Linnean Society,2011,102(3):694-706.
[31]Ergens R. Nine species of the genus Cichlidogyrus Paperna, 1960 (Monogenea:Ancyrocephalinae) from Egyptian fishes[J].Folia Parasitologica,1981,28(3):205-214.
[32]包特力根白乙.中国罗非鱼养殖产业发展及市场前景[J].中国水产,2014(9):20-22. |
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