|
|
|
| In Vitro Killing of Ciliates and Toxicity of Compound Chinese Herbal Medicines to Turbot Scophthalmus maximus |
| QIN Zhihua1, ZHANG Linlin1, GUO Pei2, RONG Rong1, ZHANG Hui1, ZHANG Lecui1, SHAN Hu1 |
1.College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China;
2.Qingdao Haixinhui Pharmaceutical Corporation Limited, Qingdao 266109, China |
|
|
|
|
Abstract Ciliates Pseudocohnilemus persalinus and Uronema marinum were held in a 96-well cell culture plate containing 90 μL of solution of 3 compound Chinese herbal medicines including Compound Ⅰ: Mahonia, Phellodendron, Scutellaria barbata, Sophora flavescens, Stemona, and Areca; Compound Ⅱ: Rheum, Scutellaria, Chrysanthemum indicum, Fructus cnidii, pomegranate peel, and mume; and Compound Ⅲ: dice, dandelion, chrysanthemum, broccoli, Patrinia, Gentian, Houttuynia, and Artemisia annua, at a dose of 0, 5, 10, 15, 20, 25, and 30 mg/kg, and 10 μL of centrifuged ciliates solution containing the ciliates at a density of 103 individuals/mL to screen the Chinese herbal medicine formulas in vitro insecticidal test by observation of the ciliates under a light microscope. Blood biochemical and histopathological indices were determined and observed in juvenile turbot Scophthalmus maximus exposed to the 3 compound Chinese herbal medicines above at a dose of 0, 20, 60, and 100 mg/kg for 7 days to evaluate toxicity of the 3 compound Chinese herbal medicines to turbot. Killing effect was observed in U. marinum exposed to Compound Ⅲ at a dose of 30 mg/kg, without mortality in Compound I solution at a dose of 0—30 mg/kg. All ciliate P. persalinus was found to be dead in the compound Ⅱgroup for 1 hour at a dose of 20, 25, and 30 mg/kg, and U. marinum was all dead in the compound Ⅱgroup for 3 hour at a dose of 30 mg/kg. There were no significant differences in blood routine indicators including white blood cells and eosinophils, as well as blood biochemical indicators such as glucose and globulin, without obvious pathological and pathological changes in the intestine, stomach, liver and spleen in each test group.
|
|
Received: 11 March 2019
|
|
|
|
|
|
[1]张永明.大菱鲆盾纤毛虫的病理学观察及中草药防治效果研究[D].青岛:中国海洋大学,2009.
[2]姚志刚.大菱鲆纤毛虫病发病机理及中药治疗技术研究[J].河北渔业,2008(8):34-36.
[3]崔龙波,刘冉,王琛,等.大菱鲆盾纤毛虫病的流行病学调查[J].科学养鱼,2015(3):54-56.
[4]方珍珍,王庆奎,陈成勋,等.复方中草药对半滑舌鳎和盾纤毛虫的急性毒性研究[J].天津农学院学报,2015,22(4):6-8.
[5]胡金城.中草药防治大菱鲆感染盾纤毛虫的技术初探[J].北京水产,2005(6):20.
[6]Munday B L, O′ Donoghue P, Watts M, et al. Fatal encephalitis due to the scuticociliate Uronema nigricans in seacaged, southern bluefin tuna Thunnus maccoyii[J]. Diseases of Aquatic Organisms,1997,30(1):17-25.
[7]徐奎栋,宋微波.海洋贝类几种危害性纤毛虫的研究[J].中国水产科学,1999,6(2):41-45.
[8]Jee B Y, Kim Y C, Park M S. Morphology and biology of parasite responsible for scuticociliatosis of cultured olive flounder Paralichthys olivaceus[J]. Diseases of Aquatic Organisms,2001,47(1):49-55.
[9]Param A, Iglesias R, lvarez M F, et al. Philasterides dicentrarchi(Ciliophora, Scuticociatida):experimental infection and possible routes of entry in farmed turbot Scophthalmus maximus[J]. Aquaculture,2003,217(1/2/3/4):73-80.
[10]汤荣成,齐红莉,王红宇,等.两种药物对褶皱臂尾轮虫和两种纤毛虫的急性毒性研究[J].水产科学,2019,38(4):539-544.
[11]沈琳琳,林能锋,詹子锋,等.不同培养条件水滴伪康纤虫种群生长研究[J].福建农业学报,2014,29(2):107-112.
[12]杨洁,刘萍,武晓玉.苦参提取物对表皮葡萄球菌的体外抗菌活性研究[J].中华医院感染学杂志,2007,17(11):1357-1358.
[13]倪小毅,陈雅棠.青蒿素衍生物抗卡氏肺孢子虫体外作用的研究[J].中华结核和呼吸杂志,2001,24(3):164-167.
[14]汪建国.渔药药效学专题讲座三[J].渔业致富指南,2017,8(2):60-64.
[15]文国樑,于明超,李卓佳,等.中草药饲料添加剂在水产养殖中的应用研究[J].广东农业科学,2011,38(24):114-116.
[16]周永灿,邢玉娜,冯全英.鱼类血细胞研究进展[J].海南大学学报(自然科学版),2003,21(2):171-176.
[17]陈洁,熊邦喜,顾钱洪,等.内源及外源因子对鱼类血液生理生化指标的影响[J].湖北农业科学,2011,50(9):1861-1865.
[18]张玉明,周健博,李明云.光唇鱼的形态特征和血液生化指标[J].浙江海洋学院学报(自然科学版),2012,31(1):59-64. |
| [1] |
SHENG Dong, REN Xu, LI Fei, HUANG Luxi, LIU Yuxiao, YU Qinghua, ZHOU Weijia, YE Shigen, GUO Zhixin. Development of an Inactivated Aeromonas salmonicida Vaccine for Turbot Scophthalmus maximus[J]. Fisheries Science, 2025, 44(3): 457-464. |
|
|
|
|
