黄颡鱼(♀)、长吻(♂)及其杂交F<sub>1</sub>代遗传多样性分析

doi:10.16378/j.cnki.1003-1111.19229

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Abstract Genetic diversity was investigated in 1 year old yellow catfish Pelteobagrus fulvidraco and longsnout catfish Leiocassis longirostris with body weight of (14.20±1.20) g and their hybrid F₁ [yellow catfish (♀) and wild longsnout catfish (♂) cross] using SSR molecular labeling technology and 12 pairs of high polymorphic primers selected from 20 pairs of microsatellite primers. The average number of allele (N_a) was shown to be 4.583 in yellow catfish, 3.667 in longsnout catfish and 5.000 in their F₁ hybrids, the average number of effective allele (N_e) was found to be 2.382 in yellow catfish, 2.221 in longsnout catfish and 2.535 in their F₁ hybrids, the average observed heterozygosity (H_o) 0.419 in yellow catfish, 0.367 in longsnout catfish and 0.604 in their F₁ hybrids, and the average expected heterozygosity (H_e) 0.477 in yellow catfish, 0.383 in longsnout catfish and 0.597 in their F₁ hybrids. There was polymorphic information content (PIC) of 0.420 in yellow catfish, 0.365 in longsnout catfish and 0.509 in their F₁ hybrids. The genetic distance between F₁ and its male parent was 0.8551, which was smaller than that between F₁ and its female parent (1.7271). The dendrogram of phylogeny revealed that the F₁ hybrids was first clustered with its male parent, showing that the hybrid F₁generation got more genetic material from the male parent and had more genetic diversity and higher gene heterozygosity than the parents. The findings provide data support for the genetic diversity of the hybrid progenies of yellow catfish and longsnout catfish and a theoretical basis for the exploration of the genetic relationship between parents and their offsprings after the intergenetic cross breeding.

Key words： Pelteobagrus fulvidraco Leiocassis longirostris genetic diversity microsatellite

Received: 21 October 2019

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	Articles by authors
	WANG Hongyu
	WU Zhaowen
	FU Dongyong
	SU Mengyuan
	YANG Wenshan
	TANG Rongye
	WANG Tao
	YIN Shaowu

Cite this article:

WANG Hongyu,WU Zhaowen,FU Dongyong, et al. Genetic Diversity of Yellow Catfish Pelteobagrus fulvidraco (♀), Longsnout Catfish Leiocassis longirostris (♂) and Their Hybrid F₁ Generation[J]. Fisheries Science, 2021, 40(2): 226-232.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.19229 OR https://www.shchkx.com/EN/Y2021/V40/I2/226

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