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| Enrichment and Release of Inorganic Arsenic in Edible Parts of Swimming Crab Portunus trituberculatus |
| ZHAO Yanfang, NING Jinsong, KANG Xuming, DING Haiyan, ZHAI Yuxiu, SHANG Derong, SHENG Xiaofeng |
| Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agricultureand Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Qingdao 266071, China |
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Abstract Mature female swimming crab Portunus trituberculatus with carapace width of (140.02±15.14) mm after temporary held were reared in a 500 L polyethylene aquarium, and exposed to 0.01 mg/L trivalent inorganic arsenic and 0.10 mg/L pentavalent inorganic arsenic prepared by mixed sodium arsenate and arsenic trioxide stock solution. The mature female swimming crab in the control group was exposed to clean and filtered fresh seawater with background inorganic arsenic content of (1.18±0.16) μg/L, and without inorganic arsenic addition. In the semi-static exposure 7-day enrichment test, the water was changed every 24 hours, and 3 samples of swimming crab were randomly taken on the 1, 3, 5, and 7 days, and the hepatopancreas, gonad and muscle tissues were immediately dissected and stored at -80 ℃ ultra-low temperature refrigerator for determination of the contents of total arsenic in 3 edible parts. In the 28-day release test, the swimming crab was exposed to natural sea water completely changed every 24 hours and 3 crab individuals were randomly sampled on the 7, 14, 21, and 28 days for determination of the contents of total arsenic in the 3 edible parts above in order to explore the accumulation and releasing of different inorganic arsenic in the edible tissues. Results showed that the bioaccumulation of inorganic arsenic was low in the three edible tissues, first increase in the content of total arsenic and then decrease in the hepatopancreas during the accumulation, without obvious change in the content of total arsenic in the muscle and gonad. During the releasing period, there was decrease in total arsenic content in the gonad and hepatopancreas, without obvious change in total arsenic content in the muscle. There were different main forms of arsenic in the three edible tissues of swimming crab in the blank control group, with arsenobetaine AsB and dimethylarsinate DMA forms in the muscle and gonad, and AsB in the hepatopancreas in the control group. The findings indicated that swimming crab had higher bioavailability of As(Ⅲ)than As(Ⅴ), and that the inorganic arsenic was mainly accumulated in the hepatopancreas, and not in the muscle and gonad.
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Received: 19 August 2019
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