复合微生物净水剂在仿刺参养殖中的应用

doi:10.16378/j.cnki.1003-1111.20074

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (2702 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract In order to study the purification capacity of the compound microbial preparations of photosynthetic bacterial Rhodopseudomonas palustris PSB1 and Bacillus BS1 isolated and preserved in the laboratory were taken as the research objects. After measuring the content changes in levels of ammonia nitrogen, and sulfide, and chemical oxygen demand (COD) in artificial sewage, the optimal density ratio of the two strains was 2∶1. Under this ratio, the ammonia nitrogen content in sewage was decreased from 4.68 mg/L to 0.39 mg/L, the sulfide content decreased from 0.62 mg/L to 0.26 mg/L, and the COD decreased from 4.02 mg/L to 2.35 mg/L. The safety test showed that the contents of arsenic and cadmium in the compound microbial water purification agent were 0.017 mg/kg and 1.634×10^-4 mg/kg, lower than the national standard, and no aflatoxin B₁, Shigella and other components were detected to meet the safety standard. In order to further explore the influence of composite microbial water purification agent on the quality of aquaculture wastewater, the microbial water purification agent was put into the sea cucumber Apostichopus japonicus culture to measure the concentration changes in COD, ammonia nitrogen and sulfide. It was found that the compound microbial water purifier had a good degradation effect on ammonia nitrogen, sulfide, and COD at pH of 7.0—8.5 and asalinity of 25—35.

Key words： photosynthetic bacteriaum Bacillus microbial water purifier Apostichopus japonicus

Received: 10 April 2020

S968.9

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	ZHOU Wenming
	LI Liying
	SUN Xinyu
	LIU Yue
	LI Ying
	LIU Bingnan

Cite this article:

ZHOU Wenming,LI Liying,SUN Xinyu, et al. Application of a Compound Microbial Water Purification Agent in Culture of Sea Cucumber Apostichopus japonicus[J]. Fisheries Science, 2021, 40(5): 686-692.

URL:

https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20074 OR https://www.shchkx.com/EN/Y2021/V40/I5/686

[1]李卓佳.华南地区对虾养殖模式与清洁健康养殖关键技术(一)[J].科学养鱼,2009(9):12-13.
[2]秦传新,张安凯,于刚,等.水产生态养殖模式研究进展[J].广东农业科学,2013,40(12):140-143.
[3]张坤,刘金凤,董琦,等.饵料中添加胶红酵母对刺参性能的影响[J].大连工业大学学报,2017,36(1):6-9.
[4]张少兵,王雅鹏.现代农业发展对环境的影响与我国的对策[J].农业现代化研究,2008,29(2):204-207.
[5]韩卓然,孙敬锋.水产动物益生菌的筛选及应用[J].水产科学,2016,35(1):93-98.
[6]孟霄鹏,孟阳,王悦,等.益生菌对凡纳滨对虾免疫功能及肠道菌群的影响[J].水产科学,2017,36(1):60-65.
[7]田良,陈政强.饲料中添加微生物制剂对仿刺参生长与免疫的影响[J].集美大学学报(自然科学版),2017,22(2):1-10.
[8]Postollec F, Mathot A G, Bernard M, et al. Tracking spore-forming bacteria in food: from natural biodiversity to selection by processes[J].International Journal of Food Microbiology,2012,158(1):1-8.
[9]贾旭颖,国先涛,王芳,等.非离子氨胁迫对淡水和海水养殖凡纳滨对虾呼吸代谢酶活力影响的比较[J].水产学报,2014,38(11):1837-1846.
[10]刘波,刘文斌.微生物对水产养殖环境的生物修复作用[J].淡水渔业,2003,33(1):50-53.
[11]Xia M S, Wang Y B, Xu Z R. The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds[J].Fisheries Science,2005,71(5):1036-1041.
[12]陈永青,林亮,杨莺莺,等.微生态制剂在水产养殖中的应用[J].生态科学,2005,24(1):80-83.
[13]陆家昌,黄翔鹄,李活,等.光合细菌对养殖水质及凡纳滨对虾抗病力的影响[J].广东海洋大学学报,2009,29(6):87-91.
[14]阮松树.介绍三种常用的水产微生态制剂[J].北京农业,2010(4):30-31.
[15]胡京,董琦,张春岩,等.两种EM菌剂对养殖水体水质及幼刺参生长性能的影响[J].大连工业大学学报,2016,35(2):79-83.
[16]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB 17378.4—2007,海洋监测规范第4部分:海水分析[S].北京:中国标准出版社,2008.
[17]中华人民共和国农业部. NY/T 1444—2007,微生物饲料添加剂技术通则[S].北京:中国农业出版社,2007.
[18]国家质量技术监督局.GB/T 17480—1998,饲料中黄曲霉毒素B₁的测定酶联免疫吸附法[S].北京:中国标准出版社,2004.
[19]中华人民共和国卫生部.GB 4789.5—2012,食品安全国家标准食品微生物学检验志贺氏菌检验[S].北京:中国标准出版社,2012.
[20]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 13092—2006,饲料中霉菌总数的测定[S].北京:中国标准出版社,2006.
[21]国家卫生和计划生育委员会,国家食品药品监督管理总局.GB 4789.10—2016,食品安全国家标准食品微生物学检验金黄色葡萄球菌检验[S].北京:中国标准出版社,2017.
[22]国家卫生和计划生育委员会,国家食品药品监督管理总局.GB 4789.3—2016,食品安全国家标准食品微生物学检验大肠菌群计数[S].北京:中国标准出版社,2017.
[23]国家市场监督管理总局,国家标准化管理委员会.GB/T 13091—2018,饲料中沙门氏菌的测定[S].北京:中国标准出版社,2018.
[24]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 13079—2006,饲料中总砷的测定[S].北京:中国标准出版社,2007.
[25]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 13081—2006,饲料中汞的测定[S].北京:中国标准出版社,2007.
[26]国家技术监督局.GB 13082—1991,饲料中镉的测定方法[S].北京:中国标准出版社,1991.
[27]国家市场监督管理总局,国家标准化管理委员会.GB/T 13080—2018,饲料中铅的测定原子吸收光谱法[S].北京:中国标准出版社,2018.
[28]任义波,温海深.微生物制剂在水产养殖中的应用[J].黑龙江水产,2009(6):23-24.
[29]孙刚,国际翔,王振堂,等.对虾杆状病毒病暴发式大流行的生态机理初步研究[J].生态学报,1999,19(2):141-144.
[30]孙冬岩,孙鸣,潘宝海.枯草芽孢杆菌对水质净化作用的研究[J].饲料研究,2009(3):58-59.
[31]沈南南,李纯厚,贾晓平,等.小球藻与芽孢杆菌对对虾养殖水质调控作用的研究[J].海洋水产研究,2008,29(2):48-52.
[32]罗勇胜,李卓佳,杨莺莺,等.光合细菌与芽孢杆菌协同净化养殖水体的研究[J].农业环境科学学报,2006,25(增刊1):206-210.
[33]姜海明,杨腾腾,冯磊,等.芽孢杆菌与光合细菌对乌鳢养殖水的净化作用研究[J].湖北农业科学,2010,49(6):1428-1430.
[34]李君华,刘佳亮,曹学彬,等.芽孢杆菌与光合细菌协同作用对养殖刺参的影响[J].渔业现代化,2013,40(1):7-12.