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| Synthesis and Antiparasitic Activity against Protozoan Parasite Ichthyophthirius multifiliis of N-Substituted-Tetrahydroisoquinoline Derivatives |
| YAO Jiayun1, ZHANG Chengsai2, YIN Wenlin1, LIN Lingyun1, PAN Xiaoyi1, CAO Zheng1, LIU Yihan1, XIA Yanchun1, SHEN Jinyu1 |
1. Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China;
2. College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety, Ministry of Education, Jilin Agricultural University, Changchun 130118, China |
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Abstract Three N-substituted-tetrahydroisoquinoline derivatives were designed, synthesized, and evaluated for their antiparasitic activity against protozoan parasite Ichthyophthirius multifiliis in vitro. The toxicity of the synthesized N-substituted-tetrahydroisoquinoline derivatives was also evaluated to develop novel antiparasitic agents against the protozoan parasite. The results showed that there was promising antiparasitic activity against I. multifiliis theronts and encysted tomonts in vitro in all the three synthesized compounds, with the maximal activity against I. multifiliis in compound 1 (2-methylamino-1,2,3,4-tetrahydroisoquinoline-formonitrile). The compound 1 had 100% effective against theronts at concentration of 20.0 mg/L in 4 h, with the median lethal concentration (LC50) of 11.6 mg/L. All dead encysted tomonts were observed in compound 1 at concentration of 40.0 mg/L, with the compound 1 48 h LC50 of 62.78 mg/L for topmouth culter Erythroculter ilishaeformis. The findings indicated that compound 1 (2-methylamino-1,2,3,4-tetrahydroisoquinoline-formonitrile) was useful in aquaculture for control of I. multifiliis due to high antiparasitic efficacy against I. multifiliis with low toxicity.
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Received: 28 October 2019
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